Differentiated cells suitable for human therapy

专利摘要:
The present invention provides a system for producing differentiated cells from a stem cell population for applications requiring a relatively homogeneous cell population. The cells contain effector genes under the control of transcriptional control elements (eg, the TERT promoter) that induce the genes to be expressed in relatively undifferentiated cells in the population. Expression of effector genes leads to expression of markers that can be used to deplete undifferentiated cells, or later to deplete them. Suitable effects and sequences encode toxins, proteins that induce apoptosis, cell surface antigens, or enzymes (eg, thymidine kinases) that convert prodrugs to lethal cells. Differentiated cell populations produced according to the present disclosure are suitable for use in tissue regeneration as well as for non- therapeutic applications such as drug screening.
公开号:KR20030081334A
申请号:KR10-2003-7007100
申请日:2001-11-26
公开日:2003-10-17
发明作者:골드조셉디；레브로스키제인에스
申请人:제론 코포레이션；
IPC主号:

专利说明:

DIFFERENTIATED CELLS SUITABLE FOR HUMAN THERAPY
[2] Cross Reference of Related Application
[3] This application was filed on November 27, 2000 and is currently ongoing. Patent applications 60 / 253,443 and 60 / 253,357; And 09 / 783,203, filed February 13, 2001 and currently in progress. For the purposes of the procedure of this application in the United States, the priority document is hereby incorporated by reference in its entirety.
[4] Background technology
[5] Precursor cells are of intense interest in medical research. Many tissues in the body have a backing reservoir of precursors that can replace cells damaged or aged by injury or disease. For use in regenerative medicine, considerable efforts have recently been made to isolate precursors of numerous different tissues.
[6] U.S. Patent 5,750,397 (Tsukamoto et al., Systemix) reports the isolation and growth of human hematopoietic stem cells that are Thy-1 +, CD34 + and can differentiate into lymphocytes, erythrocytes, and osteoblastic lineages. U.S. Patent 5,736,396 (Bruder et al.) Reported a lineage directed differentiation of isolated human mesenchymal stem cells using appropriate bioactive factors. Induced cells can then be introduced into the host for mesenchymal tissue regeneration or repair.
[7] U.S. Patent 5,716,411 (Orgill et al.) Proposes skin regeneration at burn or injury sites using epithelial allografts. U.S. Patent 5,766,948 (F. Gage) reports a method for producing neuroblast cells from animal brain tissue. U.S. Patent 5,672,499 (Anderson et al.) Reported a method for obtaining neural ridge stem cells from embryonic tissue. U.S. Patent 5,851,832 (Weiss et al., Neurospheres) reported a method for isolation of putative neural stem cells from 8-12 week old human fetuses. U.S. Patent 5,968,829 (M. Carpenter) reports human neural stem cells derived from adult primary central nervous system tissue.
[8] U.S. Patent 5,082,670 (F. Gage) reports a method of transplanting genetically modified cells to treat defects, diseases or damage of the central nervous system. Auerbach et al. (Eur. J. Neurosci. 12: 1696, 2000) reported that pluripotent CNS cells transplanted into animal brain form electrically active and functionally linked neurons. Brustle et al. (Science 285: 754, 1999) reported that precursor cells derived from embryonic stem cells interact with host neurons and efficiently myelinize axons in the brain and spine.
[9] Significant interest has arisen from the development of embryonic stem cells that are thought to have the potential to differentiate into many cell types. Initial work on embryonic stem cells was performed in mice. Mouse stem cells can be isolated from both early embryonic cells and embryonic cells. The desirable properties of pluripotent stem cells are that they can proliferate in vitro in an undifferentiated state, maintain a common karyotype, and differentiate into inducers of all three germ layers (endoderm, mesoderm and ectoderm). Is to maintain the potential.
[10] The development of human pluripotent stem cell preparations is significantly less advanced than working with mouse cells. Thomson et al. Proliferated pluripotent stem cells from lower primates (U.S. Patent 5,843,780; Proc. Natl. Acad. Sci. U.S.A 92: 7844, 1995), and then humans (Science 282: 114, 1998). Gearhart and coworkers derived human embryonic germline (hEG) cell lines from fetal germline tissue (Shamblott et al., Proc. Natl. Acad. Sci. U.S.A 95: 13726, 1998; and U.S. Patent 6,090,622).
[11] Both hES and hEG cells have long-established characteristics of pluripotent stem cells: they can grow in vitro without differentiation, have normal karyotypes, and still produce many different cell types. Clonal-derived human embryonic stem cell lines maintain proliferative pluripotency and proliferative potential for a long time in culture medium (Amit et al., Dev. Biol. 227: 271, 2000). These cells serve as a reservoir for the regeneration of almost all tissues damaged by genetic abnormalities, trauma or disease states, maintaining significant prospects for use in human therapy.
[12] International patent application publication WO 99/20741 (Geron Corp.) mentions methods and materials for the growth of primordial stem cells derived from primates. According to one embodiment, the cell culture medium is provided for growing primal stem cells derived from primates in a substantially undifferentiated state and has a low osmotic pressure and a low endotoxin level. The basal medium combines growth of primordial stem cells derived from primates and nutrient serum effective for supporting the substrate of feeder cells or extracellular matrix components derived from feeder cells. The medium may further comprise nucleosides or pyruvate salts as non-essential amino acids, antioxidants, and growth factors.
[13] A significant requirement in using stem cells for therapy is to control the differentiation and growth into specific types of tissue required for the treatment of each patient.
[14] U.S. Patent 4,959,313 (M. Taketo, Jackson Labs) provides specific enhancer sequences that induce the expression of adjacent foreign or recombinant genes from promoters that involve genes that generally do not induce expression of undifferentiated cells. U.S. Patent 5,639,618 (DA Gay, Plurion Inc.) proposes a method for isolating lineage specific stem cells in vitro, in which a pluripotent embryonic stem cell is operably linked to a reporter gene. After transfection with the linked constructs, the cells are cultured under conditions in which the cells differentiate, and then separated from other cells by isolation of reporter expressing cells.
[15] U.S. Patent 6,087,168 to Levesque et al., Cedars Sinai Med. Ctr., Relates to a method of transdifferentiating epidermal cells into viable cells useful for both cell therapy and gene therapy. Skin cells are transfected with neuronal transcription factors and cultured in a medium containing antisense oligonucleotides corresponding to negative regulators of neuronal differentiation.
[16] International Patent Publication WO 97/32025 (Mclvor et al., U. Minnesota) proposes a method for transplanting drug resistant hematopoietic stem cells. Transplanted cells are augmented by drug-directed genes (such as methotrexate resistant dihydrofolate reductase) under the control of promoters functioning in stem cells. The cells are administered to mammals, which are treated with drugs to increase the transplantation rate of transgenic cells as compared to non-transgenic cells.
[17] WO 98/39427 (Stein et al., U. Massachusetts) relates to a method for expressing a foreign gene in differentiated cells such as skeletal tissue. Stem cells (eg, bone marrow derived) are contacted with nucleic acids whose genes are linked to elements that regulate expression of differentiated cells. An example is the rat osteocalcin promoter. International Patent Publication WO 99/10535 (Llu et al., Yale U.) proposes a method for studying changes in gene expression in stem cells. The gene expression profile of the stem cell population is obtained and then compared with the gene expression profile of the differentiated cells.
[18] WO 99/19469 (Braetscher et al., Biotransplant) relates to a method of growing differentiated pluripotent embryonic stem cells from pigs. A selectable marker gene is inserted into the cell to be regulated by a control or promoter sequence in the ES cell, for example the porcine OCT-4 promoter.
[19] WO 00/15764 (Smith et al., U. Edinburgh) relates to the proliferation and induction of embryonic stem cells. The cells are cultured in the presence of a compound that selectively inhibits the proliferation or survival of cells other than ES cells by inhibiting the signal pathways essential for the differentiated cells to proliferate. Examples include compounds that inhibit the SHP-2, MEK or ras / MAPK cascades.
[20] Klug et al. (J. Clin. Invest. 98: 216, 1996) propose a strategy for genetically selecting cardiomyocytes from differentiated mouse embryonic stem cells. Fusion genes consisting of cDNA encoding α-heart myosin heavy chain promoter and aminoglycoside phosphotransferase are safely transfected into ES cells. The resulting cell lines are differentiated in vitro and selected using G418. Selected cardiomyocyte cultures are recorded as very highly differentiated. In reverse transplantation with mice, ES-derived cardiomyocyte grafts can be detected at least 7 weeks after transplantation.
[21] Schuldiner et al. (Proc. Natl. Acad. Sci. USA 97: 11307, 2000) report the effect of eight growth factors on cell differentiation from human embryonic stem cells. After initiation of differentiation through culture formation, cells were cultured in the presence of bFGF, TGF-β1, Activin-A, BMP-4, HGF, EGF, βNGF or retinoic acid. Each growth factor has a unique effect on the differentiation pathway, but no growth factor is involved in the absolute differentiation into one cell type.
[22] New approaches are needed to generate populations of differentiated cells suitable for human administration.
[1] The present invention relates generally to the field of cell biology of embryonic cells and to the field of molecular biology of viral vectors regulated by promoters. More specifically, the present invention describes a technique for removing undifferentiated cells from populations derived from pluripotent stem cells using selectively expressed soluble vectors.
[23] Summary of the invention
[24] The present invention provides a system for depleting relatively undifferentiated cells from a heterogeneous cell population that can be obtained, for example, by differentiation of stem cells. The population is treated with a vector inserted with a lethal or potentially lethal effector gene under the control of a genetic element capable of expressing the gene at higher levels in an undifferentiated subgroup. This results in a large population of comparable mature cells, which is suitable for use in regenerative medicine.
[25] One embodiment of the invention relates to a population of cells differentiated from stem cells cultured ex vivo, wherein said population is essentially free of undifferentiated cells. Examples are primitive pluripotent stem cells of primate origin, such as human embryonic stem cells.
[26] Cells in the population can be derived using polynucleotides comprising a PX structure, wherein X is a lethal to the expressing cell or a nucleic acid sequence that makes the expressing cell susceptible to a lethal effect of an external effector. ; P is a transcriptional regulatory element that induces X to be ubiquitously expressed in undifferentiated cells. The connecting line in P-X indicates that the genetic element is operably linked and whether it is neighboring within the nucleic acid molecule.
[27] X is described in the following detailed description of effector sequences. X encodes an enzyme (eg, thymidine kinase) that converts prodrugs (eg, ganciclovir) into toxins, proteins that induce or mediate apoptosis, or compounds that are lethal to cells in which X is expressed. Other examples are provided later in this disclosure.
[28] According to some embodiments, P-X is a heterologous molecule introduced, which means that the cell or its ancestral cell has been genetically modified by a vector comprising P-X. In another embodiment, it means that the cell or its ancestral cell has been genetically modified by a vector that places X under the control of an endogenous transcriptional control element. After transfection, X may be routinely found in undifferentiated cells in the population, or P-X may be inherited and expressed in undifferentiated progeny cells. Non-limiting examples for P include promoters of the OCT-4 promoter and telomerase reverse transcriptase (TERT). The cells may also contain the drug resistance gene Y under the control of P (which is referred to herein as PXY, which represents a functional relationship where P regulates the transcription of both X and Y, the elements described above). In any direction within the sequence connecting the functional moieties).
[29] Another embodiment of the invention relates to a stem cell genetically modified to contain a nucleic acid sequence having the above-described P-X structure. The invention also provides a polynucleotide vector adapted to genetically modify stem cells in the manner described above.
[30] Another embodiment of the invention is directed to a method of generating a differentiated cell population. Cell populations comprising undifferentiated stem cells containing nucleic acid molecules comprising a P-X structure are treated to induce differentiation of at least some undifferentiated cells from said population.
[31] Another embodiment is directed to a method for depleting undifferentiated stem cells from a cell population. Stem cells in the population are genetically modified such that they contain a nucleic acid sequence comprising the P-X structure described above. In this manner, genes that are lethal to the expressing cells or susceptible to the lethal effects of external agents are placed under the control of transcriptional control elements that induce unbiased expression of the gene in undifferentiated cells. If the cell population is still predominantly undifferentiated (before inducing differentiation), or if the cell population already comprises predominantly differentiated cells, the cell population may be genetically modified.
[32] When X is lethal to cells, undifferentiated stem cells can be simply depleted by culturing the cell population under conditions in which X is expressed. When X provides a cell susceptible to the lethal effect of an external agent (eg, a drug or prodrug), undifferentiated stem cells can be depleted by binding the cell with an external agent. This is done by contacting the cells with an agent in tissue culture in vitro or by administering the cells to the subject simultaneously or sequentially with an external agent (if no agent is present).
[33] The techniques and reagents of the present invention can be applied on cell populations containing any form of stem cells. They are particularly suitable for application to primate pluripotent stem cells, such as human embryonic stem cells.
[34] Other embodiments of the invention will be apparent from the detailed description below.
[35] Brief description of the drawings
[36] 1 is a feeder cell (mEF) or extracellular matrix (Matrigel), using conventional medium (RM) or conditioned medium (CM) Or laminin) for analysis of OCT-4 and hTERT expression in hES cells incubated. The top panel is a gel photograph showing OCT-4 and hTERT expression at the mRNA level by RT-PCR. The lower panel is a bar graph comparing expression levels for cells grown on different substrates, expressed as the ratio of OCT-4 or hTERT to 18s standard. Laminin and Matrigel in conditioned medium HES cells grown in the phase have an expression pattern similar to cells grown on the feeder layer.
[37] FIG. 2 is a half-tone reproduction of a gel showing telomerase activity measured in cultured hES cells by TRAP activity assay. All culture conditions showed positive telomerase activity after 40 days in feeder-free culture medium.
[38] Figure 3 is a halftone reproduction showing the expression of the GFP reporter gene in hES cells transduced with retrovirus and then differentiated. hES cells were transferred to suspension culture medium to form a culture, incubated for four more days, then replated on gelatin-coated slides, incubated for one week, then fixed and fluoresced for GFP expression. Pictures were taken below. The left panel shows bright series of illumination and the right panel shows fluorescence due to GFP expression.
[39] FIG. 4 shows the results of studies that genetically altered hES cells in feeder-free culture media by lipofection. Panel A is a halftone representation of an optical micrograph showing the morphology of hES cells on laminin after transfection. Panel B is halftone reproduction of fluorescence micrographs showing GFP expression in the same colony. Panel C is a bar graph showing the percentage of cells expressing GFP under various conditions.
[40] 5 is a map of a TPAC vector named pGRN376. This is a 7185 bp adenovirus vector comprising the herpes simplex thymidine kinase ( tk ) gene under the control of a promoter obtained from the upstream sequence of the human gene for telomerase reverse transcriptase (hTERT). Expression of tk is promoted in cells expressing hTERT, such as undifferentiated embryonic stem cells.
[41] 6 is a two panel line graph showing the effect of TPAC thymidine kinase vector on undifferentiated hES cells. After 48 hours of placement, cells were transduced with TPAC vectors of MOI 30 or 100, or transduced with mock (no vector added). After 4 hours, cells were replaced with fresh medium containing prodrug gancyclovir (GCV). On day 3, wells treated with TPAC vector + GCV contained as much as 8% of the cells in the control wells.
[42] 7 is a bar graph showing GCV titration in hES cells treated with TPAC vector. Six hours after transfection with the vector, fresh medium containing the indicated concentrations of GCV was added. About 20 μM of GCV was optimal under the test conditions.
[43] FIG. 8 is two panel bar graphs showing titration of GCV to hES cells transduced with and morphically transduced with TPAC vectors from two different cell lines.
[44] 9 shows the effect of TPAC + GCV treatment on mixed cell populations obtained from differentiation of hES cells. Cells were fed daily with conditioned medium to maintain undifferentiated state, or 500 nM of retinoic acid or 0.5% DMSO daily to induce differentiation into delegated cells of mixed phenotype. After 7 days, they were infected with TPAC vector of MOI 30 + GCV of 20 μM.
[45] The top panel is a bar graph showing the number of cells that survived in the culture medium. Treatment with TPAC + GCV removed cells cultured under each condition. In each instance, the culture of viable cells produced significantly differentiated and populations that appeared to be substantially free of undifferentiated forms. Bottom panel is halftone reproduction of gel showing RT-PCR analysis of viable cells. Cells cultured by conditioned medium (mEF-CM) or DMSO did not have detectable OCT-4 expression, and two of the four samples treated with retinoic acid (RA) were associated with very low levels of OCT-4 expression. Matched amplification products were shown.
[46] 10 is a reproduced micrograph of a hES cell line transduced by binding a control adenovirus vector (Panel A), or pGRN376 (Panel B) containing the tk gene under the control of the TERT promoter. Both wells of the transduced cells were incubated for 3 days in medium containing gancyclovir. Typical undifferentiated colonies of normal hES cell culture media were shown in control wells. In wells treated with pGRN376, most or all undifferentiated ES cell colonies were removed and only differentiated cells remained.
[47] FIG. 11 is two panel line graphs showing drug sensitivity of undifferentiated cells containing thymidine kinase gene (TPAC) induced by telomerase promoter. The top and bottom panels show sensitivity to prodrug gancyclovir (GCV) and (E) -4- (2-bromovinyl) -2'-deoxyuridine (BVDU), respectively. Gancyclovir at concentrations as low as 2.5 μM substantially eliminated all of the undifferentiated TPAC ES cells in about 4 days.
[48] 12 (A) and (B) include black and white reproduction of fluorescence micrographs of differentiated ES cells. Cell lines H9-376m-18, H9-376m-62 and H9-376m-6 contain the TPAC gene; H9-pGK-neo-1 is a control cell line transfected with only drug selection plasmids. Stably transfected cells were differentiated into cultures and mortified for immunohistochemical analysis. Three or more TPACs containing stem cell lines represent staining regions for muscle specific actin, α-fetoprotein, β-tubulin and cardiac troponin I, which are representative of all three embryonic germ layers.
[49] Detailed description of the invention
[50] Various types of stem cells have become a very interesting field in regenerative medicine. They can be propagated in culture medium and then differentiated into the cell forms required for treatment in vitro or in situ. Recently, human embryonic stem cells have been shown to sustainably express high levels of telomerase, maintaining the telomere length and growing almost indefinitely in culture media.
[51] Efforts to differentiate stem cells to date have been directed to the identification of culture conditions that facilitate the derivation of cell populations with the phenotypic characteristics of the tissue form primarily required in regenerative medicine. Schuldiner et al. (Supra) reported the effect of growth factors on the differentiation of human embryonic stem cells. U.S. In patent 5,639,613, stem cells are transfected with lineage specific genes operably linked to the reporter gene used to select cells expressing the reporter. In WO 97/32025, hematopoietic stem cells are augmented by drug resistance genes and subsequently transplanted into the subject. The cells are administered to a mammal and then treated with the drug to increase the transplantation rate of the transgenic cells. Klug et al., Supra, used a construct that regulates the expression of aminoglycoside phosphotransferase by the α-heart myosin heavy chain promoter. Transfected differentiated cells are selected using G418, which produces a cell line of cardiomyocyte type cells. This is a positive selection using gene expression patterns of the desired tissue morphology to allow biased survival of differentiated tissues.
[52] It is hypothesized that some of the populations of differentiated cells generated using the culture medium and positive selection methods employed are suboptimized for use in human therapy. In some circumstances, undifferentiated cells in the population may worsen the transplantation or function of cells in vivo. In addition, undifferentiated cells can increase the likelihood of malignancy or other tumors formed at the site of treatment transplantation or by migration of transplanted cells.
[53] The present invention relates to a strategy capable of depleting undifferentiated cells remaining in the differentiated cell population. This allows the cells to be genetically placed such that genes that are lethal to the expressing cells or that are susceptible to the lethal effects of external agents are placed under transcriptional control of the genetic elements that induce biased expression in any undifferentiated cells in the population. It is effective by deforming. This is a negative selection strategy designed to minimize the portion of undifferentiated cells. In order to obtain a relatively pure population of the desired tissue form essentially free of undifferentiated cells, the technique can be combined with various kinds of positive selection techniques.
[54] As a non-limiting validation of the invention, human embryonic stem (hES) cells are transfected with an adenovirus vector (TPAC) in which the herpes virus thymidine kinase gene is located under the control of the promoter sequence of human telomerase reverse transcriptase (hTERT). Introduce. hES cells structurally express hTERT, but this ability disappears upon differentiation. Example 10 (FIGS. 6-8) show that transduction of hES cells with TPAC vectors is likely to cause undifferentiated cells to be killed by prodrug gancyclovir at a concentration of about 20 μM, which is a substrate for thymidine kinase. Shows. Example 11 (FIG. 9) shows that when hES cells were transduced with TPAC vectors and differentiated with DMSO, there were no viable cells with detectable OCT-4 expression (phenotype of undifferentiated cells).
[55] The technology of the present invention relates, in part, to the provision of cell populations with improved properties for human treatment. After depleting undifferentiated cells, differentiated populations are expected to have better functional and transplantation properties, reducing the risk of unwanted tissue structure and malignant tumors being produced in treated patients. In addition, cell populations depleted of undifferentiated cells are more homogeneous, which provides distinct advantages for non-therapeutic applications such as producing antibody production, cDNA libraries, and screening drug candidates.
[56] Justice
[57] Circular “primate pluripotent stem cells” (pPS cells) are differentiated pluripotent cells derived from tissues of pre-embryo, embryo or fetus at any time after fertilization and, according to standard technical acceptance tests, all three germ layers (endoderm, mesoderm) And progeny of several different cell types, derivatives of ectoderm), under the appropriate conditions, such as the ability to form teratomas in 8-12 week old SCID mice.
[58] The definition of pPS cells includes various types of embryonic cells, including human embryonic stem (hES) cells (Thomson et al., Science 282: 1145, 1998); Embryonic stem cells from other primates, such as Rhesus stem cells (Thomson et al., Proc. Natl. Acad. Sci. USA 92: 7844, 1995), marmoset stem cells (Thomson et al., Biol. Reprod. 55: 254, 1996) and human embryonic germ (hEG) cells (Shamblott et al., Proc. Natl. Acad. Sci. USA 95: 13726, 1998). Other types of differentiation cells are also included in this term. Any cell of primate origin capable of producing progeny, derivatives of all three germ layers, whether derived from embryonic tissue, fetal tissue or other origin, is included. The present invention relates to pPS cells that are not derived from malignant tumor sources. It is preferred that the cells have a general karyotype (but need not always).
[59] pPS cell cultures are described as “undifferentiated” when they represent the morphological characteristics of undifferentiated cells, such that a substantial portion of stem cells and their derivatives in the population are clearly distinguished from differentiated cells of embryonic or adult origin. Undifferentiated pPS cells are readily recognized by those skilled in the art and typically exhibit a colony of cells with a high nuclear / cytoplasmic ratio and protruding phosphorus in two-dimensional microscopy. Colonies of undifferentiated cells in a population are often understood to be surrounded by differentiated neighboring cells. Nevertheless, undifferentiated colonies persist when the population is cultured or passaged under appropriate conditions, and individual undifferentiated cells make up a significant proportion of the cell population. Substantially undifferentiated cultures contain at least 20% undifferentiated pPS cells and may contain 40%, 60% or 80% undifferentiated pPS cells in increasing order of preference. Whenever a culture medium or cell population is referred to herein as "proliferating" without proliferation, this means that the composition after proliferation is substantially undifferentiated according to the above definition.
[60] "Feeder cell" or "feeder" is a term used to describe one type of cell that is co-cultured with another type of cell to provide an environment in which the second type of cell can grow. Optionally the feeder cells are derived from different species than the cells they support. For example, certain types of pPS cells, as described herein, can be supported by primary mouse embryonic fibroblasts, immortal mouse embryonic fibroblasts, or human fibroblastoid cells differentiated from hES cells. Can be. If cells grow through at least one round after splitting without adding new feeder cells to support the growth of pPS, the pPS cell population is said to be "essentially free" of feeder cells. Cultures essentially free of feeder cells contain less than about 5% feeder cells. Whenever a culture or cell population is referred to herein as being "no feeder", it means that the feeder cells are essentially free from the composition according to the above definition, which receives only the additional constraints that are explicitly required.
[61] The term "embryoid body" is a technical term with the same meaning as "aggregate bodies". These terms refer to aggregates of differentiated and undifferentiated cells that appear when pPS cells are overgrown in monolayer culture or maintained in suspension culture. Cultures are mixtures of different cell types, typically from several germ layers, which can be distinguished on a morphological basis.
[62] The terms "committed progenitor cells", "lineage restricted precursor cells" and "limited developmental lineage cells" all refer to embryonic-derived pluripotent stem cells capable of providing progeny of all three germ layers. Typically, in a more limited range, it refers to cells that can proliferate and differentiate into many different cell types. Non-limiting examples of delegated progenitor cells include hematopoietic cells that are pluripotent for various blood cells; Hepatocyte progenitors that are pluripotent for bile duct epithelial cells and hepatocytes; And mesenchymal stem cells. Another example is nerve limiting cells, which can give rise to glia cell precursors that can progress to oligodendrocytes and astrocytes, and nerve precursors to neurons.
[63] For the purposes of this specification, the term "stem cells" may refer to pluripotent stem cells or delegate progenitor cells (all defined above). At a minimum, stem cells have the ability to proliferate and form cells of one or more phenotypes, and can also self-replicate when cultured as part of the same culture or under different conditions. Embryonic stem cells can be identified positive for the enzyme telomerase.
[64] As used herein, "differentiated" and "undifferentiated" are relative terms depending on the context in which they are used. Specifically, with respect to a particular form of a particular form of self-renewing stem cells, the term "undifferentiated" refers to the same self-replicating stem cells, and the term "differentiated" refers to the morphological criteria they produce, It relates to one or more relatively mature phenotypes that stem cells can produce, which can be distinguished by antigenic markers and gene transcripts. Undifferentiated pPS cells have the ability to differentiate into all three germ layers. Cells differentiated from them can be readily recognized by those skilled in the art by morphological criteria.
[65] The terms "polynucleotide" and "nucleic acid molecule" relate to nucleotide polymers of any length. This includes genes and gene segments, mRNAs, tRNAs, rRNAs, ribozymes, cDNAs, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA and RNA, nucleic acid probes and primers. The term polynucleotide, as used herein, has the meaning interchangeable with a double- or single-stranded molecule. Unless otherwise specified or required, any embodiment of the present invention with respect to polynucleotides includes both double stranded forms and each of two complementary single stranded forms known or expected to constitute a double stranded form. This also includes nucleic acid analogs such as phosphoramidates and thiophosphoramidates.
[66] When introducing a polynucleotide into a cell by any suitable means of artificial manipulation, or when the cell is a progeny cell of the originally modified cell that inherits the polynucleotide, the cell is "genetically modified", "transfected". Or "genetically transformed." Often, polynucleotides comprise a sequenceable sequence encoding a protein of interest, which allows the cell to express elevated levels of the protein. If the progeny cells of the modified cell have the same modification, the genetic modification is said to be "genetic."
[67] A "control element" or "control sequence" is a nucleotide sequence involved in the interaction of molecules that contribute to the regulation of the function of a polynucleotide, such as replication, copying, transcription, splicing, translation or degradation of polynucleotides. Transcription control elements include promoters, enhancers, and repressors.
[68] Certain gene sequences, referred to as promoters, such as "TERT promoter" or "OCT-4 promoter" are polynucleotide sequences derived from genes that promote the transcription of operably linked gene expression products. In some cases, it is recognized that various regions of the upstream region and intron untranslated gene sequences may contribute to promoter activity, and that all or any subset of these regions may be present in the genetically engineered mentioned constructs. The promoter may be based on the gene sequence of any species having the gene, unless explicitly limited, and may incorporate any desired additions, substitutions or deletions as long as it can facilitate transcription in the target cell. Genetic constructs made for human treatment typically comprise a fraction that is at least 90% identical to the promoter sequence of the human gene. For example, activity and specificity for specific sequences can be tested by operably linking to reporter genes (Example 9).
[69] Genetic elements are referred to as "operably linked" if there is a structural relationship in which they can operate in a manner dependent on the expected function. For example, if a promoter can help initiate the transcription of a coding sequence, the coding sequence can be referred to as operably linked to the promoter (or under the control of the promoter). There may be an intervening sequence between the promoter and coding region as long as the functional relationship can be maintained.
[70] With respect to coding sequences, promoters and other genetic elements, the term “heterologous” means that the elements are derived from beings that are distinctly genetically distinct from other beings. For example, promoters or genes introduced into animals of other species by genetic engineering techniques are referred to as heterologous polynucleotides. A "endogenous" genetic element is an element that exists at the same position in a naturally occurring chromosome, even though other elements have been artificially introduced into neighboring positions.
[71] The terms "polypeptide", "peptide" and "protein" may be referred to and used herein in the same sense as amino acid polymers of any length. The polymer may comprise modified amino acids, which may be linear or branched, and non-amino acids may be involved.
[72] General technology
[73] To further elaborate general techniques useful in the practice of the present invention, one of ordinary skill in the art can consult standard textbooks and reviews in cell biology, tissue culture, and embryology. Teratocarcinomas and Embryonic Stem Cells: A Practical Approach (E. J. Robertson, IRL Press Ltd. 1987); Guide to Techniques in Mouse Development (P. M. Wasserman et al., Academic Press 1993); Embryonic Stem Cell Differentiation in Vitro (M. V. Wiles, Meth. Enzymol. 225: 900, 1993); Properties and uses of Embryonic Stem Cells: Prospects for Application to Human Biology and Gene Therapy (P. D. Rathjen et al., Reprod. Fertil. Dev. 10:31, 1998). Differentiation of stem cells is described by Robertson, Meth. Cell Biol. 75: 173, 1997; And Pedersen, Reprod. Fertil. Dev. 10:31, 1998].
[74] Methods of molecular genetics and genetic engineering are generally described in the literature: Molecular Cloning: A Journal of A Laboratory Manual (Sambrook et al.) ; Oligonucleotide Synthesis (edited by MJ Gait); Animal Cell Culture (RI Freshney Editor); Gene Transfer Vectors for Mammalian Cells (by Miller &Calos); Current Protocols in Molecular Biology an Short Protocols in Molecular Biology , 3rd edition (FM Ausubel et al., Edited); And Recombinant DNA Methodology (R. Wu, Academic Press). In this specification, reagents, cloning vectors, and kits for genetic manipulation can be purchased from commercial vendors such as BioRad, Stratagene, Invitrogen, and ClonTech.
[75] General techniques and cell collection in cell culture are summarized in the following literature: Large Scale Mammalian Cell Culture (Hu et al., Curr. Opin. Biotechnol. 8: 148, 1997); Serum-free Media (K. Kitano, Biotechnology 17: 73, 1991); Large Scale Mammalian Cell culture (Curr. Opin. Biotechnol. 2: 375, 1991); And Suspension Culture of Mammalian Cells (Birch et al., Bioprocess Technol. 19: 251, 1990). Other observations on the medium and their influence on the culture environment were performed by Marshall McLuhan and Fred Allen.
[76] Source of Stem Cells
[77] The invention can be practiced using various forms of stem cells, which can include the following non-limiting examples.
[78] U.S. Patent 5,851,832 describes pluripotent neural stem cells obtained from brain tissue. U.S. Patent 5,766,948 describes a method for producing neuroblast cells from neonatal cerebral hemispheres. U.S. Patents 5,654,183 and 5,849,553 describe the use of neuronal crest stem cells in mammals. U.S. Patent 6,040,180 describes an in vitro production method of neurons differentiated from a culture of pluripotent CNS stem cells in mammals. WO 98/50526 and WO 99/01159 describe methods for the generation and isolation of neuroepithelial stem cells, oligodendrocyte-astrocytic precursors, and lineage limiting neural precursors. U.S. Patent 5,968,829 describes neural stem cells obtained from embryonic whole brain and cultured using a medium comprising glucose, transferrin, insulin, selenium, progesterone and some other growth factors.
[79] Primary liver cell cultures can be obtained from human biopsies or surgically cut tissues by perfusion with a suitable combination of collagenase and hyaluronidase. Alternatively, EP 0 953 633 A1 describes a method for isolating liver cells by preparing human liver tissue, extracting concentrated tissue cells in culture medium, and then expanding the cells in the culture medium. Growth media include glucose, insulin, transferrin, T ₃ , FCS, and various tissue extracts from which hepatocytes can grow without transformation into malignancies. Cells in the liver are believed to contain specialized cells, including: liver parenchymal cells, Kupffer cells, sinusoidal endothelium, and cholangiovascular epithelial cells, and mature hepatocytes or Precursor cells with the ability to differentiate into bile epithelial cells (hereinafter referred to as "hepatoblasts" or "ovarian cells") (LE Rogler, Am. J. Pathol. 150: 591, 1997; M. Alison, Current Opin. Cell Biol. 10: 710, 1998; Lazaro et al., Cancer Res. 58: 514, 1998).
[80] U.S. Patent 5,192,553 describes a method for isolating human newborn or fetal hematopoietic stem or gland cells. U.S. Patent 5,716,827 describes human hematopoietic cells that are Thy-1 positive ancestral cells, and suitable growth media for their in vitro regeneration. U.S. Patent 5,635,387 describes a method and apparatus for culturing human hematopoietic cells and precursors thereof. U.S. Patent 6,015,554 describes a method for reconstituting human lymphocytes and dendritic cells.
[81] U.S. Patent 5,486,359 describes a homogeneous population of human mesenchymal stem cells that can differentiate into one or more connective tissue forms such as bone, cartilage, tendons, ligaments and dermis. They are obtained from the bone marrow or periosteum. Also described are the culture conditions used to expand mesenchymal mesenchymal cells. WO 99/01145 describes human mesenchymal stem cells isolated from the peripheral blood of an individual treated with a growth factor such as G-CSF or GM-CSF. WO 00/53795 describes stem cells and grid cells derived from adipose tissue substantially free of adipocytes and red cells. Such cells can be expanded in the literature and cultured to produce hormonal and conditioned culture media.
[82] The invention can be practiced using stem cells of any vertebrate species. This includes stem cells from humans as well as non-human primates, livestock animals, livestock animals, and other non-human mammals.
[83] Stem cells suitable for use in the present invention are primate differentiated pluripotent stem (pPS) cells derived from tissues formed after conception, such as blastocysts, or fetal or embryonic tissues obtained at any time during conception. Non-limiting examples are phylogenetic cell lines or primary cultures of embryonic stem cells or embryonic germ cells.
[84] Medium and Feeder Cells
[85] The medium for isolating and culturing pPS cells may have any of several other formulations as long as the cells obtained have the desired properties and can be further expanded. Suitable sources are as follows: Dulbecco's modified Eagles medium (DMEM), Gibco # 11965-092; Knockout Dulbecco's modified Eagles medium (KO DMEM, Gibco # 10829-018; 200 mM L-glutamine, Gibco # 15039-027; non-essential amino acid solution, Gibco 11140-050; β-mercaptoethanol, Sigma # M7522; human recombinant basis Fibroblast Growth Factor (bFGF), Gibco # 13256-029.Examples of serum containing ES medium include 80% DMEM (typically KO DMEM), defined fetal calf serum (FBS), 20 mM that is not 20% heat inactivated, 0.1 mM Prepared using non-essential amino acids, 1 mM L-glutamine, and 0.1 mM β-mercaptoethanol The media is filtered and stored for no more than two weeks at 4 ° C. The serum-free ES medium is 80% KO DMEM, Prepared using 20% serum replacement, 0.1 mM non-essential amino acid, 1 mM L-glutamine, and 0.1 mM β-mercaptoethanol The effective serum replacement is Gibco # 10828-028. Store for no more than 2 weeks at 4 ° C. Immediately before use, 4 ng / ml of human bFGF. To a final concentration of (Bodnar et al., Geron Corp, WO 99/20741).
[86] Feeder cells (if used) are grown in mEF medium containing 90% DMEM (Gibco # 11965-092), 10% FBS (Hyclone # 30071-03) and 2 mM glutamine. mEF was propagated in a T150 flask (Corning # 430825), split the cells 1: 2 once every two days using trypsin, and then kept the cells subconfluent. After preparing the feeder cell layer, irradiation is carried out at a dose (about 4000 rad gamma irradiation) that inhibits cell proliferation but allows the synthesis of factors important for supporting hES cells. A 6 well culture plate (eg Falcon # 304) was coated by incubating overnight at 37 ° C. with 1 ml of 0.5% gelatin per well and 375,000 irradiated mEFs per well were wound. The feeder cell layer is typically used for 5 hours to 4 days after tooth formation. Prior to inoculation of pPS cells, the medium was replaced with fresh hES medium.
[87] Culture conditions for other stem cells are known and can be appropriately optimized according to the cell type. Media and culture techniques for the specific cell types mentioned in the previous section are provided by reference.
[88] Embryonic stem cells
[89] Embryonic stem cells can be isolated from blastocysts of species belonging to primates (Thomson et al., Proc. Natl. Acad. Sci. U.S.A 92: 7844, 1995). Human embryonic stem cells (hES) are described in Thompson et al. (US Pat. No. 5,843,780; Science 282: 1145, 1998; Curr. Top. Dev. Biol. 38: 133 ff., 1998) and Reubinoff et al. (Nature Biotech. 18: 399, 2000) Can be prepared from human blastocyst cells using the techniques described in.
[90] Briefly, human blastocysts are obtained from human embryos prior to in vivo transplantation. Alternatively, in vitro fertilization (IVF) embryos can be used, or one cell of a human embryo can be expanded to the blastocyst stage (Bongso et al., Hum Reprod 4: 706, 1989). Human embryos are cultured up to the blast stage in G1.2 and G2.2 media (Gardner et al., Fertil. Steril. 69: 84, 1998). Developing blastocysts are selected for ES cell isolation. Zona pellucida is removed from blastocyst by brief exposure to pronase (Sigma). Internal cultures were exposed to 1:50 dilution of rabbit anti-human spleen cell serum for 30 minutes, then washed three times with DMEM for 5 minutes, and exposed to 1: 5 dilution of Guinea Completion (Gibco) for 3 minutes. Cell masses are isolated immunoimmunically (see Solter et al., Proc. Natl. Acad. Sci. USA 72: 5099, 1975). After two more washes with DMEM, lysed trophectoderm cells are removed from the intact internal cell mass (ICM) by gentle pipetting, and the ICM is placed on the mEF feeder layer. plating).
[91] 9 to 15 days later, by exposure to calcium and magnesium free phosphate buffered saline (PBS) (containing 1 mM EDTA), to dispase or trypsin, or by physical separation by micropipette Separating the derivatives induced by internal cell mass into chunks; Remount onto mEF in fresh medium. The isolated cells are placed on the mEF feeder layer in fresh ES medium and colony formation is observed. Colonies showing undifferentiated morphology are individually selected by micropipette, physically separated into chunks, and then repositioned. The ES type morphology is characterized by a high density of nuclei to the cytoplasm and dense colonies with nucleolus. Individual colonies by simple trypsin treatment, exposure to Dulbecco's PBS (without calcium and magnesium, containing 2 mM EDTA), exposure to type IV collagenase (about 200 U / ml; Gibco), or micropipettes By selection of, the resulting ES cells are usually divided every 1-2 weeks. The mass size of about 50 to 100 cells is optimal.
[92] Embryonic germ cell
[93] Human embryonic cells (hEGs) can be prepared from primordial germ cells present in human fetal formulation about 8-11 weeks after the last menstrual cycle. Suitable methods of preparation are described in Shamblott et al. (Proc. Natl. Acad. Sci. U.S. 95: 13726, 1998) and US Pat. No. 6,090,622.
[94] Briefly, the genital ridge is washed with isotonic buffer, then placed in 0.1 ml of 0.05% trypsin / 0.53 mM sodium EDTA solution (BRL) and cut to a size of 1 mm 3 or less. This tissue is then pipetted through a 100 μl tip to further deaggregate the cells. Incubate at 37 ° C. for about 5 minutes, then add about 3.5 ml of EG growth medium. EG growth medium was DMEM, 4500 mg / l D-glucose, 2200 mg / l · mM sodium bicarbonate; Fetal bovine serum (BRL) suitable for 15% ES; 2 mM glutamine (BRL); 1 mM sodium pyruvate (BRL); Human recombinant leukemia inhibitory factor (LIF, Genzyme) at 1000-2000 U / ml; Human recombinant basal fibroblast growth factor (bFGF, Genzyme) at 1-2 ng / ml; And 10 μM forskolin (present in 10% DMSO). In another approach, EG cells are isolated using hyaluronidase / collagenase / DNase. Gonadal anlagen or gonad ridges with mesentery are cut from fetal preparations, rinsed gonads with PBS, and then 0.1 mL of HCD cleavage solution (0.01% hyaluronidase type V, 0.002% DNase I, 0.1 % Collagenase type IV, all of which are commercially available from Sigma, prepared in EG growth medium. Tissues are evicted and incubated at 37 ° C. for 1 hour or overnight, then resuspended in 1-3 ml of EG growth medium and placed on feeder layers.
[95] 96-well tissue culture plates were prepared using a sub-confluent layer of feeder cells cultured in modified EG growth medium without LIF, bFGF or forskolin for 3 days, and with a γ- of 5000 rad. It is inactivated by irradiation. Suitable feeders are STO cells (ATCC Accession No. CRL 1503). About 0.2 ml primary germ cell (PGC) suspension is added to each well. The primary passage is performed for 7-10 days in EG growth medium and each well is transferred to a well of a 24-well culture dish prepared previously using irradiated STO mouse fibroblasts. The cells are incubated with changing medium daily, typically until 7-30 days or after 1-4 passages, until a cell morphology consistent with the EG cells is observed.
[96] Proliferation of Undifferentiated p-PS Cells
[97] pPS cells can be proliferated continuously during culture by using a combination of culture conditions that promote proliferation without promoting differentiation.
[98] Typically, pPS cells are cultured on feeder cells, typically fibroblastoid cells, often derived from embryonic or fetal tissue. The cell line is typically irradiated to inhibit proliferation and then implanted near the confluence used to support the pPS cell culture.
[99] In one example, pPS cells are first induced and supported on primary embryonic fibroblasts. Mouse embryo fibroblasts (mEF) can be obtained from two-crossed CF1 mice (SASCO) or other suitable strains. On the 13th day of gestation, the embryos of the mice are swabed with 70% ethanol and the decidua are transferred to phosphate buffered saline (PBS). Recover the vessel; Removal of the placenta, membranes, and soft tissues; Wash the body twice with PBS. They were then transferred to a fresh 10 cm bacterial dish containing 2 ml trypsin / EDTA and finely chopped. After 5 min incubation at 37 ° C., trypsin is inactivated with 5 ml of DMEM containing 10% fetal calf serum (FBS) and the mixture is transferred to a 15 ml conical tube and separated. The debris is allowed to settle for 2 minutes and the supernatant is made up to 10 ml of the final volume and then toothed on a 10 cm tissue culture plate or T75 flask. The flask is incubated for 24 hours without interruption, then the medium is replaced. When the flasks were confluent (about 2 to 3 days), they were split 1: 2 into new flasks.
[100] Geron scientists have found that hPS cells can remain undifferentiated without feeder cells. Environments for feederless culture include suitable culture substrates, in particular extracellular matrices that can be derived from the basal membrane or can form part of the adsorbent molecule receptor-ligand coupling. Suitable formulations are tradename Matrigel Is available from Becton Dickenson. Other extracellular matrix components and component mixtures are also suitable as an alternative. Depending on the cell morphology to be proliferated, it may comprise laminin, fibronectin, proteoglycans, entaxin, heparan sulfate, and the like, alone or in various combinations. Laminin is a major component of all basal laminae in vertebrates, which interacts with integrin heterodimers such as α6β1 and α6β4 (specific for laminin) and other heterodimers (cross-react with other matrices). do.
[101] Differentiation of pluripotent stem cells is carried out on substrates which are properly distributed in the presence of a medium which promotes cell survival and proliferation and maintains desired properties. It was found that a tooth density of about 15,000 cells / cm 2 (preferably between 90,000 cells / cm 2 and 170,000 cells / cm 2) promotes survival and limits differentiation. Passage of pPS cells in the absence of a feeder benefits by making pPS cells in small clusters. Typically enzymatic cleavage is stopped before the cells are fully dispersed (ie about 5 minutes with collagenase IV). The mass of about 10-2000 cells is then directly imbedded on the substrate without additional dispersion. Alternatively, primate PS cells can be passaged between feeder-free culture media as a higher quality cell suspension, provided that appropriate enzymes and media are selected and the densities are high enough. For example, confluent human embryonic stem cells cultured in the absence of a feeder are plated by incubating at 37 ° C. for 5-15 minutes with a solution of 0.05% (wt / v) trypsin (Gibco) and 0.053 mM EDTA. Is removed from. Pipetting is used to remove remaining cells in the plate and the cells are ground by pipetting until the cells are dispersed into a suspension containing single cells and some small clusters. The cells are then dented at a density of 50,000-200,000 cells / cm 2 to promote survival and limit differentiation. The phenotype of ES cells passaged with this technique is similar to that observed when cells are recovered as clusters by collagen cleavage. As another option, the cells can be recovered without enzymes before the plates reach confluence. The cells are incubated for about 5 minutes in a solution of 0.5 mM EDTA alone in PBS, washed out of the culture vessel and then plated in fresh culture medium without further dispersion.
[102] PPS cells dented in the absence of new feeder cells have an advantage in that they are cultured in nutrient media. The medium generally contains components for enhancing cell viability, including isotonic buffers, essential minerals, and serum or some kind of serum substitute. Irradiated primary mouse embryonic fibroblasts (or other suitable cellular preparations) at a density of about 5-6 × 10 ⁴ cells / cm 2, in serum-free medium such as 20% serum replacement and 4 ng / ml of basal fibroblast growth factor. Conditioned media can be prepared by culturing in KO DMEM supplemented with (bFGF). The culture supernatant is recovered after about 1 day at 37 ° C.
[103] As an alternative to primary mouse fibroblast culture media, conditioned media can be prepared from embryonic fibroblast cell lines tested for their ability to conditionally media. The cell line may optionally be transfected with telomerase reverse transcriptase to enhance replication capacity. Another possible source is differentiated pPS cells with the morphological properties of fibroblasts. pPS cells are suspensions cultured as aggregates in differentiation medium using non-adsorbed cell culture plates (about 2 × 10 ⁶ cells / 9.6 cm 2). After 2 days, the aggregates are transferred to a plate coated with gelatin and after about 11 days fibroblastoid cells appear in clusters of 100-1000 cells in the mixed population. After simple collagenase treatment, fibroblastoid cells can be collected under a microscope, passaged in mEF medium, and tested for their ability to condition ES medium.
[104] Conditioned medium for 1-2 days is typically used to support pPS cell culture for 1-2 days and then replaced. If desired, the conditioned medium may be supplemented prior to use with additional growth factors to aid in pPS cell culture. For hES, growth factors such as bFGF or FGF-4 can be used. For hEG, the culture medium may be supplemented with growth factors such as bFGF, inducers of gp130 such as LIF or oncostatin-M, and factors that raise cyclic AMP levels, such as forskolin.
[105] Characteristics of Undifferentiated pPS Cells
[106] On standard two-dimensional microscopy, hES cells have dense colony formation with high nuclear / cytoplasmic ratios in the plane of the plane, pluripotent, almost indistinguishable cell junctions. Cell line karyotyping can be performed using standard G-banding techniques (such as those used in numerous clinical diagnostic laboratories providing conventional karyotyping services, such as Cytogenetics Lab, Oakland, CA) compared to published human karyotypes. have.
[107] HES and hEG cells may also be characterized by expressed cell markers. In general, tissue specific markers referred to herein include suitable immunological techniques such as flow cytometry for membrane binding markers, immunohistochemistry for intracellular markers, and enzyme linked immunoassays for markers secreted into the medium. Can be detected using. In addition, expression of protein markers can be detected at the mRNA level by reverse transcriptase PCR using marker specific primers (see U.S. Patent 5,843,780 for more details).
[108] Stage specific embryonic antigen (SSEA) is characteristic of certain embryonic cell morphology. Antibodies to SSEA markers can be obtained from the Developmental Studies Hybridoma Bank (Bethesda MD). Other useful markers can be detected using antibodies against Tra-1-60 and Tra-1-81 (Andrews et al., Cell Lines from Human Germ Cell Tumors , in EJ Robertson, 1987, supra). hES cells are typically SSEA negative and SSEA-4 positive. hEG cells are typically SSEA-1 positive. Differentiation of pPS cells in vitro results in decreased SSEA-4, Tra-1-60 and Tra-1-81 expression and increased SSEA-1 expression. In addition, pPS cells can be characterized by the presence of alkaline phosphatase activity, which immobilizes the cells with 4% paraformaldehyde and then, as described in the manufacturer's instructions (Vector Laboratories, Burlingame CA), substitutes Vector Red as a substrate. It can detect by developing.
[109] Embryonic stem cells are also typically telomerase positive and OCT-4 positive. Telomerase activity can be determined using TRAP activity assays (Kim et al., Science 266: 2011, 1997), or on the market in kits XK Telomerase Detection Kit, Cat. No. s7707; Intergen Co., Purchase NY; Or TeloTAGGG ^™ telomerase PCR ELISAplus, Cat. No. 2,013,89; Roche Diagnostics, Indianapolis). HTERT expression can also be assessed at the mRNA level by RT-PCR. LightCycler TeloTAGGG ^™ hTERT Quantification Kit (Catalog 3,012,344; Roche Diagnostics) is commercially available for research purposes.
[110] Differentiation of pPS Cells
[111] Differentiation of pPS can be initiated by first forming an embryoid body. General principles for culturing cultures are O'Shea, Anat. Rec. (New Anat. 257: 323, 1999). pPS cells are cultured in a manner that allows for the formation of aggregates using a variety of choices, for example: substrates with low adhesion properties that allow overgrowth of donor pPS cell culture media, or EB formation. Cultivation of pPS in a culture vessel having. In addition, the culture may be made of suspension culture medium. pPS cells are recovered by simple collagenase cleavage, separated into clusters, and implanted in non-adherent cell culture plates. Aggregates are fed once every few days and then recovered after a suitable period of time, typically 4-8 days. The cells can then be cultured in a medium and / or on a substrate that promotes the growth of cells of a particular lineage. The substrate may be a matrix component such as Matrigel (Becton Dickenson), laminin, collagen, gelatin, or matrix producing cell lines (e.g., fibroblasts or endothelial cell lines) are first incubated, and then the matrix produced by lysis and washing in such a way that the matrix adheres to the surface of the container It may include. The culture may comprise a heterologous cell population, having an endoderm on the outside and a mesoderm and ectoderm on the inside.
[112] Geron scientists have found that pPS cells can differentiate into committed precursor cells, or finally differentiated cells without the formation of a culture, or aggregates as an intermediate step. Briefly, suspensions of undifferentiated pPS cells are prepared and then toothed on a solid surface that promotes differentiation. Suitable substrates include adherent glass or plastic surfaces. For example, the glass coverslips can be coated with a polycationic material such as polyamines such as polylysine, polyornithine, or other homogeneous or mixed polypeptides, or other polymers with predominantly positive charges. The cells are then cultured in a suitable nutrient medium adapted to promote differentiation into the desired cell lineage.
[113] In some circumstances, differentiation is further facilitated by withdrawing serum or serum replacement from the culture medium. This can be achieved, for example, by replacing with a medium free of serum and serum replacement at the time of placement. According to some embodiments of the invention, differentiation is promoted by withdrawing one or more media component (s) that promote growth of undifferentiated cells or act as inhibitors of differentiation. Examples of such components include constant growth factors, mitogen, leukocyte inhibitory factor (LIF) and basal fibroblast growth factor (bFGF). Differentiation can also be promoted by adding media components that promote differentiation into the desired cell lineage or inhibit the growth of cells with undesired properties. For example, to produce cells committed to the neuronal or glia lineage, the medium may optionally include the following factors or media components in effective combinations: neurostimulatory factors (BDNF) derived from the brain, Neurotropin-3 (NT-3), NT-4, epidermal growth factor (EGF), ciliary nerve stimulating factor (CNTF), nerve growth factor (NGF), retinoic acid (RA), sonic hedgehog , FGF-8, ascorbic acid, forskolin, fetal bovine serum (FSB), and bone morphogenic protein (BMP).
[114] General principles for obtaining tissue cells from pluripotent stem cells are reviewed in Pedersen (Reprod. Fertil. Dev. 6: 543, 1994) and US Pat. No. 6,090,622. Other interesting publications include: For neural progenitors, neural limiting cells and glia cell precursors, see Bain et al., Biochem. Biophys. Res. Commun. 200: 1252, 1994; Trojanowski et al., Exp. Neurol. 144: 92, 1997; Wojcik et al., Proc. Natl. Acad. Sci. U.S.A 90: 1305-130; And US Pat. Nos. 5,851,832, 5,928,947, 5,766,948 and 5,849,553. For cardiac muscle and cardiomyocytes, see Chen et al., Dev. See Dynamics 197: 217, 1993 and Wobus et al., Differentiation 48: 173, 1991. For hematopoietic gland cells, see Burkert et al., New Biol. 3: 698, 1991 and Biesecker et al., Exp. Hematol. See 21: 774, 1993. U.S. Patent 5,773,255 relates to glucose reactive insulin secreting pancreatic beta cell lines. U.S. Patent 5,789,246 relates to hepatocyte progenitor cells. Other interesting cells of interest are, but are not limited to, chondrocytes, osteoblasts, retinal pigment epithelial cells, fibroblasts, skin cells such as keratinocytes, dendritic cells, hair follicle cells, neural epithelial cells, smooth muscle and skeletal muscle cells, testicular gland cells And vascular endothelial cells.
[115] Geron scientists have found that culturing pPS cells or cultures in the presence of ligands that bind growth factor receptors promotes an increase in neural precursors. The growth environment may contain neuronal cell support extracellular matrix such as fibronectin. Suitable growth factors include, but are not limited to, antibodies to the receptors of EGF, bFGF, PDGF, IGF-1, and the ligand. Subsequently, the cultured cells can be optionally isolated by expression of a marker such as A2B5. Under appropriate circumstances, a population of cells expressing A2B5 markers has the ability to produce both neurons (including mature neurons) and glia cells (including astrocytes and oligodendrocytes). Optionally, the cell population can be further differentiated, for example, by culturing in a medium containing an active agent of cAMP.
[116] Geron scientists have found that culturing pPS cells or culture cells in the presence of hepatocyte differentiators promotes the increase of hepatocyte cells. Growth environment is collagen or Matrigel Hepatocyte-supported extracellular matrix, such as Suitable differentiating agents include various isomers of butyrate and analogs thereof, such as n-butyrate. Cultured cells are optionally cultured simultaneously or sequentially with the following: organic solvents such as hepatocyte maturation factors such as dimethyl sulfoxide (DMSO); Maturation cofactors such as retinoic acid; Or cytokines or hormones such as glucocorticoids, epidermal growth factor (EGF), insulin, TGF-α, TGF-β, fibroblast growth factor (FGF), heparin, hepatocyte growth factor (HGF), IL-1, IL- 6, IGF-I, IGF-II and HBGF-1.
[117] Geron scientists have also found that hPS cells can differentiate into populations containing significantly higher cardiomyocytes or cardiomyocyte precursors. Cells of the cardiomyocyte lineage can be obtained by differentiating hES cells in a growth environment that includes a cardiotrophic factor that affects DNA-methylation, which can be exemplified as 5-azacytidine, for example. Can be. The autoconstricted cells can then be separated from other cells in the population, for example by density centrifugation. Additional processes include culturing the cells in a medium containing creatine, carnitine or taurine. In addition, hPS cells can be differentiated into osteoblastic progenitor cells or osteoblasts expressing osteocalcin and collagen-1. The cells harvest mesenchymal cells derived from pPS and differentiate them in media containing bone morphogenic proteins (particularly BMP-4), ligands of human TGF-β receptors, or ligands of human vitamin D receptors. Can be obtained by
[118] Characteristics of differentiated cells
[119] Cells can be characterized according to numerous phenotypic criteria. Such criteria include, but are not limited to, morphological properties, detection and quantification of expressing cell markers, and characterization of enzymatic activity, and testing of functional properties of cells in vivo.
[120] Markers of interest in neurons include: β-tubulin III or neurofilaments that are characteristic of neurons; Glia fibrillar acidic protein (GFAP) present in astrocytes; Galactosereveloside (GalC) or myelin based protein (MBP), which is characteristic of oligodendrocytes; OST-4, which is characteristic of undifferentiated hES cells; Nestin, a hallmark of neural precursors and other cells. A2B5 and NCAM are characteristic of glia precursor cells and neural precursor cells, respectively. In addition, cells can be tested for the secretion of characteristic biologically active substances. For example, GABA-secreting neurons can be identified by the production of glutamic acid decarboxylase or GABA. Dopaminergic neurons can be identified by the production of dopa decarboxylase, dopamine or tyrosine hydroxylase.
[121] Interesting markers for liver cells include α-fetoprotein (liver follicle cells); Albumin, α ₁ -antitrypsin, glucose-6-phosphatase, cytochrome p450 activator, transferrin, asialo glycoprotein receptor, and glycogen reservoir (hepatocyte); CK7, CK19 and γ-glutamyl transferase (bile epithelial tissue). Hepatocyte differentiation has been reported to require the transcription factor HNF-α (Li et al., Genes Dev. 14: 464, 2000). Markers independent of HNF-4α expression include α ₁ -antitrypsin, α-fetoprotein, apoE, glucokinase, insulin growth factor 1 and 2, IGF-1 receptor, insulin receptor and leptin. Markers dependent on HNF-4α expression include albumin, apoAI, apoAII, apoB, apoCIII, apoCII, aldolase B, phenolalanine hydroxylase, L-type fatty acid binding protein, transferrin, retinol binding protein, erythropoietin (EPO )
[122] The cell morphology in a mixed cell population derived from pPS cells can be recognized by the characteristic morphology and the markers they express. For skeletal muscle: myoD, myogenin and myf-5. For endothelial cells: PECAM (platelet endothelial cell adsorption molecule), Flk-1, tie-1, tie-2, vascular endothelial cells (VE) caherin, MECA-32 and MEC-14.7. For smooth muscle cells: specific myosin heavy chain molecule. For cardiomyocytes: GATA-4, Nkx2.5, cardiac troponin I, α-myosin heavy chain molecule and ANF. For pancreatic cells: pdx and secreted insulin. For hematopoietic cells and their progenitor cells: GATA-1, CD34, AC133, β-major glubulin and β-major glubulin-type gene βH1.
[123] Certain tissue specific markers, as listed herein or known in the art, include immunological techniques such as flow immunocytochemistry for cell surface markers, intracellular or cell surface markers (eg, immobilized cells or tissues). Sections) immunohistochemistry, Western blot analysis of cell extracts, and enzyme-linked immunoassays on cell extracts or products secreted into the medium. In addition, the expression of tissue specific gene products can be expressed by polymerase chain polymerization (RT-) initiated by Northern blot analysis, dot blot hybridization analysis, or by reverse transcriptase using sequence specific primers in standard amplification methods. PCR) can be detected at the mRNA level. Sequence data for certain markers listed herein can be obtained from public databases such as GenBank (URL www.ncbi.nlm.nih.gov:80/entrez).
[124] Preparation of cell populations essentially free of undifferentiated cells
[125] According to the present invention, the population of differentiated cells is relatively by expressing genes that are lethal or susceptible to lethal effects of external agents, under the control of transcriptional control elements that induce genes to be biased in undifferentiated cells. Depleted undifferentiated cells.
[126] To achieve this, the effector gene is inserted before or after the process used to differentiate the cell into the lineage required for the treatment, under the control of transcriptional control elements with the required properties, suitable for negative selection of undifferentiated cells. In a manner, the cells are genetically modified.
[127] Transcription Control Factors to Induce Negative Selection
[128] Control elements are selected in terms of protein expression patterns of undifferentiated and differentiated cells in the population.
[129] Genes with the required expression patterns can be expressed by comparing expression at the level of transcription, translation or function in two different cell populations, one with relatively high numbers of differentiated cells and the other with relatively high numbers of undifferentiated cells. Can be identified. Suitable comparison methods include subtractive hybridization of cDNA libraries and microarray analysis of mRNA levels. If the transcript is identified as having an appropriate expression pattern, a promoter or enhancer of the corresponding gene can be used for construction of the negative selection vector.
[130] Suitable microarray analysis is performed using a Genetic Microsystems array generator and Axon GenePix ^™ scanner. Microarrays are prepared by amplifying cDNA in 96 or 384 well format and then spotting directly onto glass slides. To compare mRNA samples from two populations of cells, one sample converts to Cy3-labeled cDNA while the other converts to Cy5-labeled cDNA. Two cDNA samples are hybridized simultaneously to a microarray slide and then washed to remove nonspecific binding. Spots that appear randomly on the array will bind to each of the cDNA products of the transcript-rich portion in the two original mRNA samples. The slides are then scanned at the appropriate wavelength for each label and the relatively abundant mRNA is detected. Preferably, the expression level of the effector gene will be at least 5 times and even 25 times higher in undifferentiated cells compared to differentiated cells.
[131] For depletion of pluripotent embryonic cells, an example of a control element is a promoter for telomerase reverse transcriptase (TERT). The sequence of the human TERT gene (including upstream promoter sequence) is provided below. Also, U.K. as a reference publication. Patent GB 2321642 B (Cech et al., Geron Corporation and U. Colorado), International Patent Publication WO 00/46355 (Morin et al., Geron Coporation), WO 99/33998 (Hagen et al., Bayer Aktiengensellschaft), and Horikawa I., et al. Cancer Res., 59: 826, 1999). The sequence of the mouse TERT gene is provided in WO 99/27113 (Morin et al., Geron Corporation). A lambda phage clone named λGΦ5 containing about 13,500 bases upstream from the hTERT coding sequence is available from ATCC (Accession No. 98505). Example 9 illustrates the testing and use of the TERT promoter sequence (SEQ ID NO: 1) in a vector expression system.
[132] Another example of a control element is the promoter sequence for Octomer binding transcription factor 4 (OCT-4), belonging to the POU family of transcription factors. OCT-4 transcription is activated between 4 and 80 cell stages in embryonic development and is highly expressed in blastocyst expansion and subsequent pluripotent cells in egg cylinders. Transcription is downregulated as primitive ectoderm differentiation to form mesoderm and limits migration of primordial germ cells about 8.5 days after sexual intercourse. In addition, high levels of OCT-4 gene expression are observed in pluripotent embryo carcinoma and embryonic stem cell lines, and when differentiation of these cells is induced, it is downregulated. Porcine, mouse and human OCT-4 promoter sequences are provided in WO 99/19469 (Biotransplant Inc.).
[133] Other suitable control elements can be obtained from genes that induce the expression of markers that are characteristic of undifferentiated cells in the population but not differentiated cells. For example, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 are characteristic of various types of undifferentiated, pluripotent embryonic stem cells. Enzymes involved in the synthesis of SSEA-4 may have transcriptional control elements with the required expression specificity. A more recent example is the promoter for Rex1 protein, a zinc finger protein regulated by retinoic acid expressed in embryos before transplantation. The mouse Rex1 promoter has been shown to act as an effective transcription marker for undifferentiated embryonic stem cells (Eiges et al., Current Biol. 11: 514, 2001).
[134] Suitability of certain elements can be assessed, for example, by analysis of gene transcript expression by microarray analysis. Then, a promoter or enhancer sequence derived from the identified cell specific gene, which controls transcription of a reporter gene such as green fluorescent protein, secretory alkaline phosphatase, β-glucuronidase or β-galactosidase Using, reporter constructs can be tested for appropriate specificity in differentiated and undifferentiated cells. The use of reporter constructs to test promoter specificity is illustrated in FIG. 9 below.
[135] Negative Selection Effect Factor Genes
[136] Transcriptional regulatory elements with appropriate specificity are operably linked to the coding region for the product that provides for the removal of expressing cells (either directly or by the cells under the action of non-toxic external agents).
[137] Suitable effector genes include peptide toxins (e.g., lysine, abrin, diphtheria, gelonin, Pseudomonas endotoxin A, crotalus durissus terripicus toxin, crotalus adamenteus toxin, nazanaza toxin and naza mocam (Hughes et al., Hum. Exp. Toxicol. 15: 443, 1996; Rosenblum et al., Cancer Immunol. Immunother. 42: 115, 1996; Rodriguez et al., Prostate 34: 259, 1998). Mauceri et al., Cancer Res. 56: 4311; 1996.
[138] Also suitable are genes that induce or mediate apoptosides (eg, the ICE family of cysteine proteases, the Bcl-2 family of proteins, Bax, bclX and caspase) (Favrot et al., Gene Ther. 5: 728, 1998; McGill et al., Front. Biosci. 2: D253, 1997; McDonnell et al., Semin, Cancer Biol. 6: 53, 1995). Another possible antitumor agent is apoptin as a protein that induces apoptosides even when small drug chemotherapy fails (Pietersen et al., Adv. Exp. Med. Biol. 465: 153, 2000). Koga et al. (Hu. Gene Ther. 11: 1397, 2000) propose telomerase specific gene therapy using an hTERT gene promoter linked to apoptoside gene caspase-8 (FLICE). Gu et al. (Cancer Res. 60: 5359, 2000) reported a binary adenoviral system that induces Bax expression via the hTERT promoter. They found that binary adenovirus systems induce tumor specific apoptosis in vitro and inhibit tumor growth in nude mice.
[139] Also of interest are enzymes present in soluble packages that deliver cytotoxic T lymphocytes or LAK cells to their target sites. Perforin and Fas ligand, the pore forming proteins, are the major cytolytic molecules in the cells described above (Brandau et al., Clin. Cancer Res. 6: 3729, 2000; Cruz et al., Br. J. Cancer 81: 881, 1999 ). In addition, CTL expresses a family of at least 11 serine proteases called granzymes with four primary substrate specificities (Kam et al., Biochim. Biophys. Acta 1477: 307, 2000). Low concentrations of streptolysin O and pneumolysin promote Granzyme B dependent apoptosis (Browne et al., Mol. Cell Biol. 19: 8604, 1999).
[140] Other suitable effectors themselves are not toxic to the cell, but provide sensitivity to other non-toxic compounds in the cell (by metabolizing the cell or transforming the non-toxic prodrug into a lethal drug). To encode a polypeptide. Lethality for progeny with undifferentiated phenotypes only occurs when prodrugs are present. Thus, prodrugs can be combined with the cells to minimize the proportion of cells with an undifferentiated phenotype while being differentiated, expanded or maintained in vitro. In addition, the reader will readily recognize that the prodrug may be provided to a patient treated with the cell to minimize the appearance of progeny with an undifferentiated phenotype in vivo, either concurrently with or after the treatment.
[141] Examples of effector genes having these properties encode thymidine kinases ( tk ), such as may be derived from herpes simplex virus, and catalytically equivalent variants. HSV tk converts the anti-herpes agonist gancyclovir (GCV) into toxic products that interfere with DNA replication in proliferating cells.
[142] US Patent 5,631,236 (Baylor Collage of Medicine) summarizes adenovirus vectors containing the HSV tk gene operably linked to a promoter that expresses the tk gene in cancer cells. US Pat. No. 5,997,859 and EP 702084 B1 (Chiron) are directed to replication deficient recombinant retroviruses carrying vector constructs which induce the expression of the HSV tk gene for converting other inactivating compounds into cytotoxic forms. EP 415731 A1, EP 657540 A1, and EP 657541 A1 (Wellcome Foundation), VZV tk , carboxypeptidase G2, alkaline phosphatase, penicillin V amidase, and cytosine to convert prodrugs into agents toxic to cancer cells. A retroviral vector encoding an enzyme, such as a deaminase, is proposed. International patent publications WO 98/14593 and WO 00/46355 (Geron Corporation) describe constructs comprising HSV tk under the control of the hTERT promoter sequence.
[143] The human HSV tk gene sequence is provided below, illustrating the use for targeting cells expressing TERT (SEQ ID NOs: 2 and 3). Simultaneously or after expression of the gene in the target cell, a switchable prodrug, such as gancyclovir, is added to the environment to effect depletion of the target.
[144] Another form of effector that makes a cell susceptible to other nontoxic agents is a gene that induces the presentation of foreign antigens on the cell membrane. The substances presented may be alloantigens, xenoantigens, or antigens from non-mammalian species from which specific antibodies are readily available. Expression of the gene induces the presentation of the antigen on undifferentiated cells, which is appropriate for immunological isolation (eg, immunoaffinity (eg, panning), fluorescence-activated cell sorting, or complement mediated lysis). It can be used to effectively deplete by.
[145] If the transduction agent is a viral vector, the effector may be a viral gene that requires replication of the virus. Genes essential for replication of adenoviruses include the E4, E1a, E1b and E2 regions. Genes essential for the replication of HSV-1 include ICP6 and ICP4. The gene is located under the control of specific promoters and is used to transduce cells in the differentiated cell population. The virus then specifically replicates any undifferentiated cells present and disrupts them. For a description of soluble vectors that replicate in cells expressing TERT, see WO 00/46355 (Morin et al., Geron Corporation).
[146] Another form of effector sequence encodes a membrane protein containing an epitope recognized by a specific antibody. The membrane protein may be a protein expressed on cells of different types of the same species, but more typically it is obtained from another species or is an artificial sequence. In this case, the antigen will be foreign to the species from which the stem cell is derived and antibodies made from the same species will not cross react with other antigens against that cell.
[147] Alternatively, the target antigen may be a cell surface carbohydrate or lipid component. In this case, the effector sequence will encode an enzyme involved in antigen synthesis. Of particular interest are glycosyl transferases of mammalian or non-mammalian origin, which synthesize novel determinants detectable by carbohydrate differentiation antigens, alloantigens, genoantigens or antibodies. Examples include marker SSEA-1, wherein the effector sequence encodes the corresponding fucosyltransferase; Galα (1,3) Gal linkers present on the endothelial tissue of most mammals except humans and ancient monkeys and formed by α (1,3) galactosyltransferase (α1,3GT); And ABO tissue hematopoiesis present on most human cells whose coding sequence is the corresponding ABO transferase (see GenBank Accession Numbers S71333, J05175 and AF134414). Both Galα (1,3) Gal and ABO determinants are prone to lysis mediated by antibodies that occur naturally in patients who do not have determinants as self antigens.
[148] Another possible effector sequence is based on RNA-interference (RNAi) technology. Double-stranded or hairpin RNAs corresponding to portions of mature mRNA (eg, gene transcripts) in the vesicles induce the target mRNA to be destroyed (Sharp et al., Genes Dev. 13: 139, 1999; Wianny et al., Nat. Cell Biol. 2: 70, 2000). For example, hairpin RNA (a transcript consisting of inverted repeats obtained from coding regions of cellular genes and separated by short linker sequences to produce synthetic RNA containing double stranded regions) Plasmids containing promoters that induce expression have been used to induce stable and heritable RNAi effects in C. elegans (Tavernarakis et al; Nat. Genet. 24: 180, 2000).
[149] According to some embodiments of the invention, the control element inducing transcription of undifferentiated cells (or cells expressing TERT) is operably linked to the coding sequence for hairpin RNAi targeting a particular gene transcript. Target genes essential for cell viability are selected: for example, basic transcription or translation factors, tRNA genes, ribosomal RNA subunits, or DNA or RNA polymerases. In one example, the hTERT promoter induces RNAi to inactivate genes in the furin recovery pathway. In the presence of drugs such as aminopterin, the new synthesis of purine is inhibited because the activity of the enzymes hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and thymidine kinase (TK) is inhibited. If the medium is supplemented with hydroxyxanthin and thymidine (HAT medium), cells with HGPRT and TK survive under aminopterin. Residual undifferentiated cells can be removed from the population by culturing with HAT medium. In addition, transcripts of essential genes such as HGPRT and TK can be targeted using other forms of effector sequences (e.g., coding sequences for major negative analogs lacking antisense polynucleotides, ribozymes, or functional catalytic domains). Can be.
[150] The effector regions used in the vectors of the invention can be constructed to be functionally controlled by molecular switches. Fusion between the ligand binding domains of the receptor results in molecules in which the general function of the original protein is inhibited in the absence of hormones recognized by the ligand binding domain. The binding of a ligand (eg, a small molecule hapten) to a binding domain unmasks or activates the effector domain, thereby constructing a fusion protein comprising a switch molecule that binds the domain coupled to the effector domain. . Suitable ligand binding domains can be obtained from receptors (eg, estrogen receptors) or antibodies. The effector domain may be selected from any of the already listed proteins that are lethal to the cell, such as peptide toxins, endonucleases (meganucleases such as I-Sce-1 or humanized forms of standard list nucleases), or apoptosis. It can be a mediator. If no ligand is present, the lethal function of the effector gene is inactive. This is another system in which the effector gene provides cells that are susceptible to the toxic effects of external agents (in this case ligands), which can be administered for the purpose of controlling the depletion of undifferentiated cells in culture medium or in vivo. To provide.
[151] In addition, vector constructs for use in the present invention may contain antibiotic resistance genes that are also present under the control of positive selection markers, such as specific promoters. An example is a vector with a batch of hTERT promoter- tk gene-IRES-neo. It is designed such that both suicide effectors and drug resistance genes are expressed under the control of the TERT promoter. Internal ribosome entry site (IRES) sequences may allow the tk gene and the neo gene to be under transcriptional control of the hTERT promoter. Post-translational cleavage sites such as the 2A sequence (Felipe et al., Gene Ther. 6: 198, 1999) can be used for similar effects. Generation and selection of hES cell lines with these stable integrated constructs is readily performed by the activity of drug resistant genes of undifferentiated cells. This has the advantage over the cotransfection method using the drug resistance gene under the control of other promoters since the drug resistance gene is not expressed in undifferentiated cells. This avoids the host's unwanted immunological response to the gene product of the transplanted cell.
[152] Selection technology for the removal of undifferentiated cells
[153] In order to deplete undifferentiated cells from the differentiated cell population, the effector genes are selectively expressed in undifferentiated cells.
[154] This can be accomplished in several ways. In one embodiment, the population is genetically modified using a vector in which a transcriptional control element with appropriate specificity is operably linked to an effector gene. Genetic modifications may be transient (eg, using adenoviruses), which means that expression levels decrease when cells divide. It is suitable for generating differentiated cell populations free of heterologous genes upon treatment. In addition, genetic modifications can be permanent (using a retroviral vector), which means that the modifications are heritable to the offspring of early modified cells. It is suitable for creating a differentiated cell population with an ongoing corrective function when propagated in vitro or in vivo to eliminate any undifferentiated or dedifferentiated cells that have increased in the population.
[155] Any suitable expression vector can be used. Viral vector systems suitable for producing stem cells modified according to the present invention can be prepared using commercially available viral components. Viral vectors containing effector genes are generally described in the publications mentioned in the last section. In addition, vector plasmids can be introduced into cells by electroporation, or using lipid / DNA complexes, as described in US Pat. No. 5,587,475; 5,627,175; 5,705,308; 5,744,335; 5,976,567; 6,020,202; And 6,051,429. For example, there is a Lipofectamine 2000 ^™ formulation available from Gibco / Life Technologies. Another exemplary reagent is FuGENE ^™ Transfection Reagent, a combination in non-liposomal form and other compounds in 80% ethanol, available from Roche Diagnostics Corporation.
[156] According to another embodiment, the effector gene is located under the control of an endogenous transcriptional control element, such as the hTERT or OCT-4 promoter. This uses, for example, a vector comprising an effector coding sequence located on the side of the transcriptional control element and the upstream region genomic sequence and on the other hand the side of the downstream region genomic sequence for the target gene. Thus, the effect can be obtained by homologous recombination. U.S. Patents 5,464,764 and 5,631,153 describe a dual selection strategy that positions two sequences homologous to a gene target to the side of the positive selection marker and attaches the negative selection marker to the 3 'end of the secondary lateral region. U.S. Patent 5,789,215 describes the use of homologous recombinant target vectors to modify the cellular genome of mouse embryonic stem cells. Other interesting information on homologous recombination targeting can be found in U.S. Patents 5,589,369, 5,776,774 and 5,789,215.
[157] If the effector gene directly induces cell lysis or apoptosis, undifferentiated cells will be depleted from the population when the cells are cultured under conditions in which the control element is expected to induce transcription of the gene. However, if the effector gene is not directly lethal, but provides a cell susceptible to the lethal effect of the external agent, the depletion will be delayed until the external agent is supplied. For example, if the gene is a prodrug converting enzyme, depletion has an effect when placing the cell in the environment containing the prodrug. If the gene is an antibody target, depletion is effective when placing the cell in an environment containing the particular antibody and complement. The environment may be a culture vessel, in which case the agent may be added directly to the culture medium at the required concentration. In addition, depletion can be performed in vivo by administering a population of cells to a patient and simultaneously or sequentially administering an agent (if not present).
[158] Cell populations in which most cells have been differentiated can be genetically modified according to the procedures described above to deplete undifferentiated cells. Alternatively, precursor populations of relatively undifferentiated cells can be genetically modified and differentiated according to the procedures described above. In such situations, more typically, effector genes are used that do not immediately kill cells upon expression but provide cells that are susceptible to the lethal effects of some external agents. In one example, undifferentiated pPS cells grown in culture medium are transduced with a retroviral vector whose herpes thymidine kinase gene is under the control of the hTERT promoter. By incorporating a selective marker into the construct or by measuring the expression of the transduced gene, positive transduced cells are optionally selected and propagated in culture medium. If differentiated cells are desired, the population is obtained through differentiation procedures (eg, to make hepatocytes or neural precursors, as mentioned above). They are then cultured under conditions that allow expression of the tk gene in the presence of gancyclovir.
[159] As an example where RNAi is an effector sequence, hES cells are stably transfected (eg, by lipofection) with a construct consisting of two cassettes: one in which the PGK promoter induces the neomycin phosphotransferase gene. (Which results in resistance to toxic neophysin derivatives such as geneticin); The other is that the hTERT promoter derives the coding region for RNAi containing a double stranded region from HGPRT or TK. Stablely modified clones are either in the medium containing geneticin and azaguanine or 6-mercaptopurin (to select HGPRT negative cells), or geneticin and (to select TK negative cells) 5 -Is isolated in a medium containing bromodeoxyuridine. Alternatively, transfection can be performed with only RNAi kits, in which case geneticin is omitted from the medium. After isolation of viable clones, the cell lines are exposed to HAT medium to induce differentiation into the desired cell morphology and to remove any residual cycle cells.
[160] Cell populations can be obtained using the above technique for "depletion" of undifferentiated cells, which exhibits any significant reduction in the percentage of undifferentiated cells present. After performing the procedure effectively, the percentage of undifferentiated cells can be reduced by 50%, even 90%. Depending on the selected control element and effector, a differentiated cell population may be obtained that is “essentially free of” undifferentiated cells. This means that the entire population contains less than 1% of cells with an undifferentiated phenotype. Populations containing 0.2%, 0.05%, 0.01%, 20 ppm or 5 ppm of undifferentiated cells are increasingly more preferred. For pPS cells, the presence of undifferentiated cells can be measured by counting SSEA-4 expressing cells by FACS analysis or by counting TERT or OCT-4 expressing cells by fluorescence in situ hybridization.
[161] Use of differentiated cells
[162] Cells prepared according to the present invention can be used for a variety of commercially important research, diagnostic and therapeutic purposes.
[163] Since the cell populations of the present invention are depleted of undifferentiated cells, they can be used to prepare antibodies and cDNAs specific for the differentiated phenotype. General techniques in the induction, purification and modification of antibodies, and their use in immunoassay and immunoisolation methods are described in the Handbook of Experimental Immunology (Weir &Blakwell); Current Protocols in Immunology (edited by Colingan et al.); And Methods of Immunolgical Analysis (Masseyeff et al., Weinheim: VCH Verlags GmbH). General techniques related to the construction of mRNA and cDNA libraries are described in the following literature: RNA Methodologies: A Laboratory Guide for Isolation and Characterization (RE Farell, Academic Press, 1998); cDNA Library Protocols (Cowell & Austin, Humana Press); And Functional Genomics (Hunt & Livesey, 2000).
[164] Relatively homogeneous cell populations are suitable for use in drug screening and therapeutic applications.
[165] Drug screening
[166] Differentiated pPS cells of the invention can be used to screen for factors (eg, solvents, small molecule drugs, peptides, polynucleotides, etc.) or environmental conditions (eg, culture conditions or manipulations) that affect the characteristics of the differentiated cells.
[167] In some applications, differentiated cells are used to screen for factors that can promote maturation or promote proliferation and maintenance of the cells in long term culture. For example, candidates for maturation factors or growth factors can be examined by adding them to pPS cells in several wells and then determining any phenotypic changes produced according to the criteria required during further culture and use of the cells. do.
[168] Specific screening applications of the present invention relate to the examination of pharmaceutical compounds in drug research. Reference publications generally refer to standard textbooks "in vitro Methods in Pharmaceutical Research", Academic Press, 1997, and U.S. Patent 5,030,015 is mentioned. Evaluation of the activity of a pharmaceutical candidate compound generally involves combining differentiated cells of the present invention with the candidate compound and altering the morphology, marker phenotype, or metabolic activity of the cells attributable to the compound (untreated cells or inactive compounds). Comparison with treated cells) and then correlating the effects of the compound by the observed changes.
[169] For example, screening can be performed because the compound is designed to have a pharmacological effect on certain cell types, or because a compound designed to have other effects may have unintended side effects. In order to detect possible drug-drug interaction effects, two or more drugs may be used in combination (simultaneously or sequentially). In some applications, compounds are initially screened for their potential toxicity (Castell et al., Pp. 375-410, "In vitro Methods in Pharmaceutical Research", Academic Press, 1997). Cytotoxicity may first be determined by cell viability, survival, morphology, and effect on the expression or secretion of certain markers, receptors or enzymes. The effect of the drug on chromosome DNA can be determined by measuring DNA synthesis or repair. In particular, the level required for [ ³ H] thymidine or BrdU incorporation, or for cell replication, at unplanned points in the cell cycle is consistent with drug effects. Unwanted effects also include the unusual rate of sister chromatid exchange as measured by diffusion in the middle phase. As a reference publication for further understanding, A. Vickers (PP 375-410, "in vitro Methods in Pharmaceutical Research", Academic Press, 1997) is mentioned.
[170] Therapeutic use
[171] Differentiated cells of the invention can also be used for tissue reconstitution or regeneration in human patients in need. The cells are administered in such a way that they can be transplanted to the tissue site of interest and reconstruct or regenerate functionally deficient areas.
[172] In one embodiment, neural stem cells are directly implanted into the parenchymal or intravesicular region of the central nervous system, depending on the disease to be treated. Transplantation is performed using single cell suspensions or small aggregates of density 25,000-500,000 cells / μl (U.S. Patent 5,968,829). The efficacy of neuronal cell implants can be assessed in a rat model for severely injured spinal cord, as described by McDonald et al. (Nat. Med. 8: 1410, 1999). Successful transplants are cells derived from implants that appear 2-5 weeks after injury and differentiate into astrocytes, oligodendrocytes and / or neurons and migrate along the spinal cord from the wound end, and improve gate, orientation and weight gain Will indicate.
[173] Some neural gland cells implemented in the present invention are designed for the treatment of acute or chronic damage to the nervous system. For example, excitotoxicity is associated with a variety of diseases including: epilepsy, stroke, ischemia, Huntington's disease, Parkinson's disease and Alzheimer's disease. Some differentiated cells of the present invention may be suitable for the treatment of dysmyelination diseases such as Felizeus-Merzbacher disease, multiple sclerosis, leukodystrophy, neuritis and neurological disorders. In order to promote remyelination, cell cultures rich in oligodendrocytes or oligodendrocyte precursors are suitable for this purpose.
[174] Hepatocytes and hepatocyte precursors prepared according to the present invention can be evaluated in animal models for their ability to repair liver damage. One such example is damage caused by intraperitoneal injection of D-galactosamine (Dabeva et al., Am. J. Pathol. 143: 1606, 1993). The efficacy of treatment can be determined by immunohistochemical staining for hepatic cell markers, microscopic determination of whether bovine structures are formed in growth tissue, and therapeutic ability to restore the synthesis of liver specific proteins. Liver cells can be used as part of a bioassist device that provides instantaneous liver function as the liver tissue of the patient regenerates itself after treatment by direct administration, or after fulminant hepatic failure.
[175] The efficacy of cardiomyocytes according to the present invention can be evaluated in animal models for cardiac cryoinjury where 55% of left ventricular wall tissue is induced into scar tissue without treatment (Li et al., Ann. Thorac. Surg. 62: 654, 1996; Sakai et al., Ann. Thorac. Surg. 8: 2074, 1999, Sakai et al., J. Thorac. Cardiovasc. Surg. 118: 715, 1999). Successful treatment will reduce scar area, limit scar expansion, and improve cardiac function as measured by cardiac contractility, cardiac pressure and developed pressure. In addition, cardiac injury can be modeled using a color coil in the distal portion of the left anterior descending artery (Watanabe et al., Cell Transplant. 7: 239, 1998), and the efficacy of the treatment is histology and heart. Can be evaluated by function. Cardiomyocyte specimens embodied in the present invention can be used in therapies to regenerate cardiac muscle and treat insufficient cardiac function (U.S. Patent 5,918,449 and WO 99/03973).
[176] The following examples are provided to illustrate the present invention in more detail, but are not meant to imply any limitation in practicing the claimed invention.
[177] Example 1: Feederless Passage of hES Cells
[178] In this example undifferentiated hES cells retained on primary mouse embryonic feeder cells were harvested and then maintained in the absence of feeder. Matrigel culture wells And cells were cultured in the presence of a suitable nutrient medium obtained from cultures of irradiated primary fibroblasts.
[179] Preparation of Conditioned Media (CM) from Primary Mouse Embryonic Fibroblasts (mEF):
[180] Fibroblasts were recovered from the T150 flask by one wash with PBS without Ca ⁺⁺ / Mg ⁺⁺ and incubated for about 5 minutes in 1.5-2 mL trypsin / EDTA (Gibco). After fibroblasts were detached from the flasks, they were collected in mEF medium (DMEM + 10% FBS). Cells were irradiated at 4000 rad (508 seconds at 140 kV: shelf setting 6 in Terrex ^™ generator), counted and inoculated with about 55,000 cells / cm 2 in EF medium (525,000 cells / well, 6 well plates). After at least 4 hours, the medium was replaced with SR (containing bFGF) containing ES medium using 3-4 mL per 9.6 cm well of a 6 well plate. Conditioned medium was collected daily for feeding of hES cultures. Alternatively, media was prepared using mEF toothed in culture flasks and replaced daily with 0.3-0.4 ml / cm 2 of media. Prior to addition to the hES culture, the conditioned medium was supplemented with 4 ng / ml human bFGF (Gibco). Fibroblast cultures were used in the system for about 1 week before replacing with freshly prepared cells.
[181] Matrigelcoating:
[182] Matrigel Reduces Growth Factors Or conventional Matrigel (Becton-Dikinson, Bedford MA) was dissolved at 4 ° C. Matrigel Was diluted 1:10 to 1: 500 (typically 1:30) with cold KO DMEM. 0.75-1.0 mL of solution was added to each of 9.6 cm 2 wells and incubated for 1 hour at room temperature. Before adding the cells, the coated wells were washed once with cold KO DMEM. The plates were used within 2 hours after coating or stored at 4 ° C. in DMEM and used within about 1 week.
[183] Human ES Culture:
[184] Undifferentiated hES colonies were recovered from hES culture medium on the following feeder. Cultures were incubated at 37 ° C. for about 5 minutes in about 200 U / ml collagenase IV. Colonies were recovered by picking individual colonies under a microscope using 20 μl pipette tips, or by scraping and separating into small clusters in conditioned medium (CM). The cells were then Matrigel in conditioned medium Phases were inoculated with 15 colonies for each 9.6 cm 2 well (if there were about 10,000 cells in one colony, the tooth density was about 15,000 cells / cm 2).
[185] Matrigel One day after inoculation onto the hES cells were visualized as small colonies (about 100-2,000 cells) and single cells were present in the colonies that appeared to be differentiated or stained. As proliferated hES cells, colonies become very large and dense and occupy most of the surface area of the culture dish. HES cells in colonies had a high cytoplasmic nuclear ratio and prominent phosphorus, similar to hES cells retained on feeder cells. At confluence, differentiated cells in colonies appeared less than 10% of the cells in culture.
[186] After 6 days of inoculation, the cultures were nearly confluent. Cultures were partitioned by incubating at 37 ° C. for about 5 minutes using 1 ml of a collagenase IV solution of about 200 U / ml in KO DMEM. The collagenase solution was aspirated, 2 ml of hES medium was added to each well, and then hES cells were scraped from the dish using a pipette. The cell suspension was transferred to 15 ml conical tubes, brought to 6 ml, and gently triturated to separate the cells into small clusters of 10-2000 cells. Subsequently, as described above, the cells were transferred to Matrigel in CM. Re-inoculation on coated plates. The cells were seeded at a ratio of 1: 3 to 1: 6, approximately 90,000 to 170,000 cells / cm 2, and the volume of each well was 3 ml. The medium was changed daily, the cells were split and then sub passaged 13 and 19 days after the initial inoculation.
[187] 19 days after the initial inoculation, cells were harvested and surface marker expression was assessed by immunofluorescence cell cytometry, using labeled antibodies specific for cell surface markers. For hES cells maintained in the absence of the feeder, high expression rates were seen for SSEA-4, Tra-1-60 or Tra-1-81. These three markers are expressed on undifferentiated human hES cells maintained on the feeder (Thomson et al., 1998). In addition, SSEA-1, a glycolipid that is not expressed (or expressed at low levels) on undifferentiated ES cells, is expressed very little. Immunohistochemical evaluations for SSEA-4, Tra-1-60 and Tra-1-81 indicate that expression of these markers is localized in ES colonies and not in differentiated cells in colonies.
[188] Cultures of hES cells were grown without apparent change in proliferative capacity or phenotype in the absence of feeder cells for at least 180 days after initial inoculation. Matrigel in mEF conditioned medium Human ES cells maintained in the phase have a doubling time of about 31 to 33 hours, similar to the rate of proliferation of hES cells grown on mEF feeder cells. H1 cells after 64 days of culture without feeder showed a general karyotype.
[189] Example 2: Phenotypic Markers of hES Cells in Feeder-Free Cultures
[190] Undifferentiated hES cells express SSEA-4, Tra-1-60, Tra-1-81, OCT-4 and hTERT. Expression of these markers is reduced upon differentiation. To test whether cells maintained under feeder-free conditions could retain these markers, cells were evaluated by immunostaining, reverse transcription PCR amplification, and telomerase activity assays.
[191] For analysis by fluorescence activated cell sorting (FACS), hES cells were separated in 0.5 mM EDTA in PBS and 5 × 10 ⁵ cells were resuspended in 50 μl dilution containing 0.1% BSA in PBS. To analyze surface marker expression, diluted IgG isotype control (0.5 μg / test), IgM isotype control (1:10), SSEA-1 (1:10), SSEA-4 (1:20) Cells were incubated at 4 ° C. for 30 minutes in primary antibodies comprising, Tra-1-60 (1:40) and Tra-1-81 (1:80). After washing with dilution, cells were incubated with PE conjugated rat anti-mouse kappa chain antibody (Becton Dickinson, San Jose, Calif.) At 4 ° C. for 30 minutes. Cells were washed and analyzed using CellQuest ^™ software on a FACScalibur ^™ flow cytometer (Becton Dickinson, San Jose, Calif.).
[192] Similar to hES cells on the feeder, Matrigel Cells on, laminin, fibronectin or collagen IV expressed SSEA-1, Tra-1-60 and Tra-1-81. SSEA-1, a glycolipid not expressed in undifferentiated hES cells, was expressed very low.
[193] For analysis by immunocytochemistry, SSEA-1 (1:10), SSEA-4 (1:20), Tra-1-60 (1:40) and Tra-1- diluted with knockout DMEM Cells were incubated at 37 ° C. for 30 minutes with primary antibody containing 81 (1:80). Cells were then washed with warm knockout DMEM and fixed for 2 minutes with 2% paraformaldehyde. After washing with PBS, cells were incubated with 5% goat serum in PBS for 30 minutes at room temperature and then for 30 minutes at room temperature with FITC-conjugated goat anti-mouse antibody (1: 125) (Sigma). Incubated. Cells were washed, stained with DAPI and then mounted. Staining was typically performed about 2 days after passage. The cells were also examined for expression of alkaline phosphatase, a marker for undifferentiated ES cells. This was done by culturing the cells on the chamber slides, fixing for 4 minutes with 4% paraformaldehyde and then washing with PBS. Cells were then incubated with phosphatase substrate (Vector Laboratories, Inc., Burlingame, Calif.) In the dark at room temperature for 1 hour. The slides were rinsed for 2-5 minutes with 100% ethanol before mounting.
[194] As a result, SSEA-4, Tra-1-60, Tra-1-81 and alkaline phosphatase, Matrigel, as seen in the cells on the feeder Or by hES colonies on laminin, but not by differentiated cells in colonies.
[195] 1 shows OCT-4 and hTERT expression of H1 cells on or without feeder, detected by reverse transcriptase PCR amplification. For radiometric relative quantification of individual gene products, QuantumRNA ^™ Alternate 18S internal standard primer (Ambion, Austin TX, USA) was used according to the manufacturer's instructions. Briefly, the linear range of specific primer pair amplifications was measured and then co-amplified with an appropriate mixture of alternative 18S primers: competimer to obtain PCR products that matched the linear range. . Prior to adding AmpliTaq ^™ (Roche) to the PCR reaction solution, the enzyme was preincubated with TaqStart ^™ antibody (ProMega) according to the manufacturer's instructions. Reflective PCR reactions were analyzed on a 5% non-modified polyacrylamide gel and exposed to phosphoimage screen (Molecular Dynamics) for 1 hour. Screens were scanned with Molecular Dynamics Storm 860 and band intensities were quantified with ImageQuant ^™ software. The results are expressed as the ratio of radioactivity incorporated into the hTERT or OCT-4 bands and normalized to radioactivity incorporated into the 18s band.
[196] Primers and amplification conditions for specific markers are as follows. OCT-4: sense (SEQ ID NO: 4) 5'-CTTGCTGCAG AAGTGGGTGG AGGAA-3 ', antisense (SEQ ID NO: 5) 5'-CTGCAGTGTG GGTTTCGGGC A-3'; Alternative 18: compiltimer = 1: 4; 19 cycles (94 ° C. 30 sec; 60 ° C. 30 sec; 72 ° C. 30 sec). hTERT: sense (SEQ ID NO: 6) 5'-CGGAAGAGTG TCTGGAGCAA-3 ', antisense (SEQ ID NO: 7) 5'-GGATGAAGCG GAGTCTGGA-3'; Alternative 18: compiltimer = 1: 12; 34 cycles (94 ° C. 30 sec; 60 ° C. 30 sec; 72 ° C. 30 sec).
[197] hTERT and OCT-4 expression in Matrigel And for all culture conditions except conventional media. In addition, after exposing the cells to retinoic acid (RA) or dimethyl sulfoxide (DMSO), the expression of hTERT, a factor promoting cell differentiation, was significantly reduced.
[198] 2 shows telomerase activity measured by TRAP activity test (Kim et al., Science 266: 2011, 1997; Weinrich et al., Nature Genetics 17: 498, 1997). All culture conditions were Matrigel in mEF conditioned medium. After 40 days on, laminin, fibronectin or collagen IV, it showed positive telomerase activity.
[199] Example 3: Differentiation of hES Cells
[200] In this example, the differentiation using the aggregate formation standard method was compared with the direct differentiation technique.
[201] For the aggregate differentiation technique, monolayer cultures of rhesus monkey and human ES cell lines were recovered by incubating for 5-20 minutes in collagenase IV, and cells were scraped from the plate. Cells were then separated and implanted into non-adherent cell culture plates in FBS containing medium. This plate was placed in a 37 ° C. incubator and in some cases a rocker was used to facilitate maintenance of aggregates in the suspension. After 4-8 days in suspension, aggregate bodies were formed and mortared on more differentiated substrates.
[202] For the direct differentiation technique, suspensions of rhesus monkey and human ES cells were prepared in a similar manner. The cells were then separated into clusters of about 50-100 cells by grinding and teethed on glass coverslips treated with poly-ornithine. Cells were maintained for 7-10 days prior to analysis in serum containing media or defined media.
[203] Cells from both specimens were fixed and tested for immune responsiveness to β-tubulin III and MAP-2, which are characteristic of neurons, and to glia fibrillar acidic protein (GFAP), which is characteristic of astrocytes. The results are shown in Table 1.
[204] Comparison of hPS Differentiation Methods ES cell line used for differentiationDifferentiation through Aggregate BodyDirect differentiation NeuronsAstrocytesNeuronsAstrocytes R366.4Rhesus monkey cell line++++ R278.5Rhesus monkey cell line++++ R456Rhesus monkey cell line++++ H9Rhesus monkey cell line++++ H9.1H9 cloneNot differentiatedNot differentiated++ H9.2H9 clone++++
[205] Using aggregate or direct differentiation techniques, rhesus monkey and human ES cell lines were differentiated into cells with markers for neurons and astrocytes. In rhesus monkey culture media, the percentage of aggregates containing neurons was between 49% and 93%. In the human cell lines examined, the percentage of aggregates containing neurons was 60% to 90%. Double labeling for GABA and β-tubulin showed that subgroups of neurons express the inhibitory neurotransmitter GABA. In addition, astrocytes and oligodendrocytes were identified as GFAP immune reactivity and GalC immune reactivity, respectively. Thus, human and rhesus monkey ES cells have the ability to form all three major cell phenotypes of the central nervous system.
[206] The effects of several components of the neurotrophin growth factor family were examined. hES cells were harvested with collagenase, isolated and differentiated by re-inoculation on slip coated with poly-ornithine. The cells were plated overnight in DMEM / F12 + N2 + 10% FBS. The next day, serum was removed from the culture medium and replaced with 10 ng / ml of human bFGF and the growth factor to be tested. After 24 hours, bFGF was removed from the medium. The culture medium was fed every 2 days. They were fixed 7 days after differentiation and immunostained for analysis. Cells positive for β-tubulin were counted to assess the number of neurons. Cultures maintained in the presence of 10 ng / ml of brain derived neurostimulatory factor (BDNF) formed about three times more neurons than control cultures. Cultures maintained under neurotropin-3 (1 ng / ml) formed about 2 times more neurons than controls.
[207] In subsequent experiments, suspensions of human ES cells were prepared from parental cell line H9 and two subclonal cell lines. Cells were harvested using collagenase IV and then remounted on glass slides coated with poly-ornithine in medium containing 20% FBS. The culture medium was then fed every 2 days for 7-10 days and then fixed for immunostaining. From each of these cell lines, numerous differentiated cells stained positive for muscle specific actin (antibody from Dako) but negative for cardiac Troponin I. Several patches of cells were stained positive for α-fetoprotein, indicating the presence of endoderm cells.
[208] Example 4: Comparison of Direct Differentiation and Differentiation Through Cultures
[209] To induce direct differentiation, undifferentiated hES cells were harvested and directly reimposed on differentiation conditions. Although significant cell death was seen in mortality, many cells began to adsorb and proliferate and / or differentiate. In culture media differentiated using serum containing conditions, the culture was continued to multiply and reach confluence within 5-10 days. At this point, the culture contained heterogeneous populations representing many different forms. By immunocytochemistry, ectoderm, mesoderm and endoderm lineages were extracted using antibodies against β-tubulin III, muscle specific actin and α-fetoprotein, respectively. Positive staining for all of these cell types was often found in very dense patches, thus making it difficult to accurately quantify the percentage of each cell type.
[210] To increase the percentage of neurons, hES cells were dentured on glass coverslips coated with poly-ornithine and cultures in defined media. The results indicate that cardiomyocytes are not identified, although cells from all three germ layers can be induced without production of EB.
[211] As a comparison, hES cells induced differentiation by producing cultures (EBs). In these experiments, ES cells were harvested and placed in suspension culture medium. Although a significant amount of cell death was observed initially, the remaining cells formed aggregates after 2-3 days. EBs were maintained for 16 days in the culture medium, but continued to survive and formed many structures after subsequent teeth. Subsequent stages of human EB often showed cyst morphology, often causing beating EB.
[212] To assess the formation of cardiomyocytes, after 4 days in suspension culture medium, EBs were transferred to gelatin coated plates or chapter slides. After inoculation, EBs were adhered to the surface, expanded, and then differentiated into other types of cells. Automatically contacting cells were observed in cultures of various areas on day 8 of differentiation, and the number of beating areas increased until about 10 days. In some cases, at least 75% of the EBs had contact areas. The beating cells were morphologically similar to the beating cardiomyocytes derived from mouse ES cells. In addition, expression of cardiac troponin I, a cardiac specific marker, was examined at day 15 of differentiation using immunocytochemistry. Individual contact focuses in the differentiated cultures were photographed and the contact area recorded before the cultures were fixed. Cultures were then assessed for cardiac cTnl expression and matched with the original photographs to determine the percentage of contact area that was positive for cTnl staining. As a control, cTnl staining was examined for cells adjacent to the contact focal point. In this culture, 100% of the contact areas showed positive immunoreactivity and minimal immunity was observed in non-biting cells.
[213] For cultures of differentiated EBs, Western blot analysis was performed using monoclonal antibodies against cTnl. The test showed a strong 31 kDa protein signal corresponding to purified negative human heart Tnl. CTnl was detected in differentiated human ES cells containing contact cells, but not in differentiated cells without evidence of undifferentiated ES cells or contact cells, suggesting specific detection of cardiomyocytes. As a control, reprobed with β-actin specific antibody to confirm that similar amounts of protein were present in all samples.
[214] In other experiments, EBs were cultured for 8 or 16 days and maintained in adherent culture for an additional 10 days. RNA was prepared from differentiated ES cells and semiquantitative RT-PCR was performed to detect relative expression of endoderm specific products α ₁ -anti-trypsin, AFP and albumin. Low levels of α ₁ -anti-trypsin and AFP were detected in undifferentiated cultures; Albumin was slightly or not detected in the same culture. All three markers were detected at significantly higher levels after differentiation. The expression of all three endoderm markers was higher in cultures derived from day 8 cultures than from day 16 cultures.
[215] Example 5: Transfection and Transduction of hES Cells Retained on the Primary mEF Feeder Layer
[216] hES cultures with 80% KO DMEM (Gibco) and 20% serum substitutes (Gibco) supplemented with 1% non-essential amino acids, 1 mM glutamine, 0.1 mM β-mercaptoethanol and 4 ng / ml hbFGF (Gibco) Maintained in the configured growth medium.
[217] Prior to the addition of the cells, the plates were coated overnight at 37 ° C. with 0.5% gelatin solution (Sigma). Primary mEFs were incubated in standard mEF medium and split 1: 2 every 2 days for up to 5 splits. Subconfluent cultures of mEF were detached with trypsin, resuspended in 10 ml of medium, and irradiated with a cumulative dose of 3500-4000 rad using a Torrex ^™ 150D X-ray generator. Irradiated cells were pelleted at 400 × g for 5 minutes and resuspended in standard mEF medium at 1.25 × 10 ⁵ cells / mL. Individual wells of 6 well plates were inoculated with 3.75 × 10 ⁵ irradiated mEFs per well; Individual wells of 24 well plates were inoculated with 75,000 irradiated mEFs per well.
[218] Transfection was performed as follows. By collagenase (about 200 U / mL) hES cells densified in 6-well plates were separated from the feeder layer at 37 ° C. for 7-10 minutes. When colonies began to detach, collagenase was aspirated from each well and replaced with 2 ml of standard hES growth medium / well. hES cells were separated from each well by scraping the surface of a single well using a 5 ml pipette and transferred to a 50 ml conical tube. Additional hES culture medium was added at 10 ml final volume. The cell suspension was milled 10-12 times using a 10 ml pipette and additional 8 ml of standard hES growth medium was added. To each well of a 6 well plate precoated with the gelatin and mEF feeder layers, 3 ml of cell suspension was added (ie 1 well of 6 well plates was sufficient to inoculate 6 wells of new plate).
[219] To determine whether genetic modification of hES cells can be achieved without causing differentiation, the repositioned hES cells were examined using a number of different transfection systems. The systems tested included: mammalian transfection kit (CaPO4 and DEAE reagents), Stratagen catalog # 200285; TranlT-LT1 Mirus ^™ (Panvera), catalog # MIR 2310; Polybrene (Sigma); Poly-L-lysine (Sigma); Superfect ^™ (Qiagen); Effectene ^™ (Quagen); Lipofectin ^™ from Life Technologies; Lipofectamine (unlike Lipofectamine 2000 ^™ ) (Life Technologies); Cellfectin ^™ from Life Technologies; DMRIE-C from Life Technologies; Lipofectamine 2000 (Life Technologies); And electroporation using BioRad ^™ gene pulsar.
[220] Under the conditions used, both Lipofectamine 2000 ^TM (Gibco Life Technologies catalog # 11668019, patent pending) and FuGENE ^TM (trade name of Fugent LLC; proprietary blend of lipids and other ingredients, purchased from Roche Diagnostic Corporation catalog # 1 814 443) It resulted in good transfection efficiency. The efficiency is generally best when the above reagents are contacted with the intact hES cells about 48 hours after the intact.
[221] Transfection with Lipofectamine 2000 ^™ was performed as follows: Plasmid DNA (3-5 μg pEGFP-C1, ClonTech Catalog # 6084-1) was diluted with water to 100 μl final volume. In a pilot experiment, 5-30 μl of Lipofectamine 2000 ^™ (Gibco, Catalog # 11668-019) was diluted with OptiMEM ^™ (Gibco, Catalog # 11-58-021) to a final volume of 100 μl. The DNA solution was then added slowly to the Lipofectamine 2000 ^™ solution and mixed gently. The mixture was incubated for 20-30 minutes at room temperature before supplementing with 800 μl OptiMEM ^™ . Cells were washed with 3 ml of pre-warmed OptiMEM ^™ and incubated at 37 ° C. for 4 hours in 0.5-1 ml of DNA / lipid mixture solution per well (9.6 cm 2). In some experiments, the complex was removed at 4 hours and then 4 ml of mEF conditioned medium was added; In another experiment, sufficient mEF conditioned medium was added to the wells to a final volume of 3.5 ml and the mixture was left on the cells overnight. In another experiment, the DNA / lipid mixture was added to a well containing enough mEF conditioned medium to reach a final volume of 3.5 ml and cells were incubated overnight in the mixture.
[222] Transfection with FuGENE ^™ was performed as follows: Using FuGENE ^™ 6 (Roche Diagnostics Corp.), 10 μg each well in a ratio of 3: 2 FuGENE ^™ reagent to DNA, according to the manufacturer's instructions. DNA was transfected. OpiMEM ^™ serum free medium was used for transfection. In the "conventional protocol", 4 hours after the addition of the FuGENE ^™ -DNA complex, 2.5 ml of standard hES growth medium was added to each transfection well. In the modified protocol ("3: 2 L"), the transfected wells were not resupplied with standard hES growth medium. 24 hours after transfection, GFP expression was assessed by flow cytometry.
[223] 48 hours before transfection, hES cells were seeded on 6-well plates coated with gelatin and mEF Peter layers as described above. hES cells were transfected using FuGENE ^™ 6 (Roche) or Lipofectamine 2000 ^™ (Gibco) according to the manufacturer's instructions. Twenty four hours after transfection, cells were assessed for GFP expression by testing under fluorescence microscopy or flow cytometry. In an experiment shown in Figure 1, to were compared in three ways: standard Lipofectamine 2000 ^TM protocol, a modified FuGENE ^TM protocol results left overnight standard FuGENE ^TM protocol, and the DNA / lipid mixture on the cell, Lipofectamine 2000 ^{It has been shown that TM} produces consistently higher percentages of GFP expressing cells, while modified FuGENE ^TM protocols result in GFP expressing cells with higher average fluorescence intensity.
[224] Transient transduction with adenovirus vectors was performed as follows. The vector Ad5CMV5-GFP (hereinafter referred to as Ad5GFP) contains the coding region of the green fluorescent protein under the control of the CMV promoter, which was purchased from Quantum Biotechnologies, catalog # ADV0030. 72 hours prior to transduction, hES cells were seeded on 24 well plates coated with gelatin and mEF feeder layers as described above. Prior to transduction, three wells of hES cells were detached at 37 ° C. with 0.05% trypsin / 5 mM EDTA (Sigma) solution, resuspended in 500 μl of standard mEF growth medium and counted with a hemocytometer (75,000 mEF feeder). Cells were removed from each well), and the cell number was checked before transfection. Adenovirus stocks immediately dissolved on ice before use.
[225] For infection with Ad5GFP, growth medium was aspirated from wells containing hES cells and replaced with 1 ml hES growth medium + 9 μl of Ad5 GFP stock (MOI 40). After 2 hours, the virus containing medium was replaced with 1 ml of hES growth medium per well. In each transduction well, 1 ml of fresh hES growth medium was resupplied every 24 hours. GFP expression was assessed by flow cytometer. As a result from a typical experiment, the expression was highest after 24 hours of transduction, but the low level persisted for at least 8 days (afterwards, excessive differentiation occurs due to overgrowth of hES cells).
[226] Example 6 Preparation of Immortal Feeder Cell Line NH190
[227] In this experiment, permanent mouse cell lines suitable for conditioned medium for the culture of primate differentiated pluripotent stem (pPS) cells were generated. NHG190 cell line is a mouse embryo fibroblast cell line that is immortalized by telomerase, which has three drug resistances and expresses green fluorescent protein (GFP).
[228] Two mouse strains with transgenes resistant to the antibiotic neomycin or hygromycin were obtained from Jackson Laboratories (Bar Harbor, Maine). C57BL / 6J TgN (pPGKneobpA) 3 Ems mice and C57BL / 6J-TgN (pPWL512hyg) 1 Ems mice from Jackson laboratory were crossed. Embryos bearing both neomycin and hygromycin resistance are standard protocols for preparing mouse embryonic fibroblasts (mEF) for feeder layers (EJ Robertson, pp. 71-112, Teratocarcinoma and Embryonic Stem Cell Lines, edited by EJ Robertson, Oxford) : IRL Press, 1987), was cut 15 days after implantation. Induced mEF cells were cryopreserved.
[229] MEF was dissolved in growth medium containing 20% fetal calf serum (HyClone), 2 mM L-glutamine (Gibco / BRL), 80% DMEM (Gibco / BRL). Cells were expanded for 4 passages using a split ratio of 1: 2. Four hours prior to electroporation, fresh medium was fed to two flasks that reached about 75% confluence. Cells were separated from the flasks using 0.5% trypsin / 500 mM EDTA (Gibco / BRL), pelleted at 400 × g at room temperature for 5 minutes, and resuspended in growth medium at a concentration of 4 × 10 ⁶ cells / ml. It was.
[230] The cell suspension was divided into two 500 μl aliquots and transferred to two 0.4 cm spacing electroporation cuvettes (BioRad). One cuvette contained 5 μg of control plasmid (pBS212; puromycin resistance gene induced by SV40 early enhancer / promoter); In another cuvette, 5 μg of pGRN190 containing the murine telomerase reverse transcriptase (mTERT) coding region induced by the MPSV promoter and the furomycin resistance gene induced by the SV40 early enhancer / promoter. Cells and DNA were mixed manually and electroporated using a BioRad gene Pulser using a BioRad capacitance extender set at 300 V, 960 μF.
[231] Each aliquot of cells was transferred to individual 150 cm plates containing 25 ml of growth medium. The medium on the plate was replaced the next day and the next day growth medium was replaced with growth medium + 0.5 μg / ml puromycin. The medium on the plate was replaced every 48 hours with fresh puromycin-containing medium until day 29 after electroporation. At this point, large individual colonies of puromycin resistant cells were evident in both pBS212 and pGRN190 electroporated plates. Ten colonies from the control plate and twelve colonies from the pGRN190 electroporated plate were isolated by cloning cylinder and each colony was transferred to one well of 48 well plates (one well per colony).
[232] After 1 week, all surviving colonies (3 control colonies, 1 pGRN190 electroporated colonies) expanded to reach confluence within 48 well plates were individually transferred to wells of 24 well plates. After 6 days, the only colonies that continued to expand were derived from pGRN190 electroporated plates, which were then named NH190 . These cells were maintained in growth medium + 0.5 μg / ml puromycin. Analysis of telomerase activity by TRAP test (Kim et al., Nucleic Acids Res. 25: 2595, 1997) showed that NH190 cells exhibited functional telomerase activity.
[233] In order to facilitate monitoring of cells in the mixed culture population and in vivo, NH190 cells were further infected with retroviral rescues that confer expression of green fluorescent protein (GFP). The enhanced GFP sequence from plasmid pEGFP-1 is one of Living Colors ^™ fluorescent protein vectors (available from ClonTech). It contains an enhanced GFP coding region that has modified the retraction nuclease cleavage site and shifted the excitation and emission wavelengths of the encoded protein. The EGFP-1 sequence was cloned into vector pMSCV.neo (ClonTech catalog # K1062-1). The designed vector was transduced into NH190 cells and GFP positive cells were isolated by FACS sorting. The GFP expressing cell line was called NHG190 . These cells were loaded in culture medium for at least 3 months.
[234] Example 7: Genetic Modification of hES Cells Retained on NHG190 Feeder Cells
[235] NGH190 cells were incubated in DMEM (Gibco) + 20% fetal bovine serum (HyClone) and 5 mM glutamine. Cells were divided into 1:10 every 3 days. Subconfluence cultures were detached with trypsin, suspended in 10 ml of medium and then irradiated with a cumulative dose of 3500 rad by a Torrex ^™ 150D X-ray generator. Irradiated cells were pelleted at 400 × g for 5 minutes and resuspended in NHG190 medium or standard hES medium at 1.25 × 10 ⁵ cells / mL.
[236] Conditioned medium was prepared by emulsifying 4.08 × 10 ⁴ / cm NHG190 cells on a plate coated with gelatin. 18-24 hours after tooth dentification, 4 ng / ml was replaced with standard hES medium with bFGF. The medium was conditioned by the cells for 18-24 hours, recovered, and then additional 4 ng / ml of bFGF added. The medium was used to support hES cells on the same day of collection. Irradiated NHG190 cells can be used to prepare conditioned medium for 7-10 days.
[237] hES cells were transfected as follows. Cells were separated from the feeder layer using collagenase (about 200 U / ml) at 37 ° C. for 7-10 minutes and transferred to 50 ml conical tubes. hES growth medium is added to a final volume of 10 ml; The suspension was ground 10-12 times using a 10 ml pipette and then another 8 ml of hES medium was added. Matrigel 3 ml cell suspension And to each well in a 6 well plate precoated with NHG190 feeder cells.
[238] 48 hours after inoculation, hES cells were transfected with 10 μg of DNA per well in OptiMEM ^™ serum-free medium using FuGENE ^™ 6 (Roche) according to the manufacturer's instructions. DNA was a plasmid containing neo ^r induced by the pGK promoter. After 4 hours, 3 ml of NHG190 conditioned medium was added to each transfection well. The cells were replenished daily with 3 ml of conditioned medium. 48 hours after transfection, cells were plated with NHG190 conditioned medium containing Geneticin (Sigma) added at 200 μg / ml and the conditioned medium was then replaced daily. After 3 days of selection, additional irradiated NHG190 feeder cells were added (1.25 × 10 ⁵ cells / well in hES medium). After 24 hours, the medium was replaced again with NGH190 conditioned medium containing 200 μg / mL of geneticin and then daily.
[239] Individual colonies were isolated and expanded through another selection process. After 5 more days, individual colonies were identified by microscope and marked on the outside of the dish. The medium was removed and replaced with collagenase (about 200 U / ml). Individual colonies were picked using a p20 pipette tip and transferred to individual tubes containing 2 ml of NHG190 conditioned medium (without geneticin). By grinding the suspension 5 times. Colonies were deagglomerated and the contents of each tube transferred to wells of a 12 well plate coated with geneticin and irradiated NHG190 cells (1.875 × 10 ⁵ cells / well). After 24 hours, cells were fed 2 ml of fresh conditioned medium. After 2 days of inoculation, the cells were laid with 2 ml of conditioned medium containing 200 μg / ml of geneticin and the medium was changed daily for 5 days. When each well reached 50-75% of the confluent, the cells were detached with collagenase, transferred to 6 ml of conditioned medium and then crushed 10-12 times. 3 ml of cell suspension is added to each of 2 wells of a 6 well plate coated with gelatin and irradiated NHG190 cells (3.75 × 10 ⁵ cells / well); At 24 hours, the cells were reloaded with 3 ml of conditioning. Cells were then selected for 5 days using 3 ml of conditioned medium containing geneticin and split 1: 6 as above.
[240] Stable transduction with retroviruses was performed as follows. The retroviral vector, named GRN354, was obtained from Geron Corp. using PMSCVneo vector (Catalog # K1062-1) purchased from ClonTech. Was built on. The eGFP coding region was inserted downstream from the MSCV LTR. LTR induces expression of GFP and the vector also contains a neo ^r gene that is induced by the murine PGK promoter. Plates were encoded with 0.5% gelatin and NHG190 feeder cells (4.5 × 10 ^{4 in} 1 ml NHG190 medium for 24 well plates; 3.75 × 10 ^{5 in} 3 ml medium for 6 well plates). hES cell line H7 was seeded on plates (1 mL / well) prepared in 24 wells in hES medium. After 48 h, 0.05% trypsin / 5 mM EDTA (Sigma) was used at 37 ° C. to detach 3 wells of hES cells, resuspend in 500 μl of NHG190 medium and count. The stock solution of retroviral construct pGRN354 was immediately dissolved on ice before use. Growth medium was aspirated from the wells and replaced with 400 μl hES medium + 8 μl retrovirus (MOI 10) and 4 μl 8 mg / ml polybrene solution (Sigma). After 2 hours, 800 μl of hES growth medium was added per well. Each transduced well was reloaded with 1 ml fresh hES medium every 24 hours.
[241] After 4 days of transduction, the medium was replaced with 1 ml of hES growth medium containing 200 μg / ml of Geneticin. After 3 days of geneticin selection, the serotos were desorbed with collagenase, ground, resuspended in 3 ml of hES medium, and re-inoculated into one well of a 6 well plate coated with gelatin and NHG190 feeder. HES medium was replenished 24 hours later. The medium was then replaced again with hES medium containing geneticin and then reloaded every 24 hours. Undifferentiated colonies survived the selection and lasted for at least 3 months. FACS analysis showed low levels, even though 50-65% of the selected cells expressed GFP. The karyotype of these cells was common.
[242] 3 shows GFP expression of hES cells transduced and differentiated by retrovirus. hES cell line H7 was wound on a drug resistant (NGH190) feeder layer, infected with GRN354 and selected for resistance to drug G418. The introduced cells were expanded and maintained under G418 selection for multiple passages. Cells were transferred to suspension cultures to form cultures, which were differentiated for 4 days, and then plated in 20% FBS medium for 1 week. After expanded differentiation, cultures were fixed with 4% paraformaldehyde and photographed under fluorescence for GFP expression. Many differentiated cells expressed higher levels of GFP than undifferentiated transfected hES cell lines, consistent with the differentiation activity of MESV-LTR in other cell types.
[243] Example 8: Transfection of Feederless hES Cells
[244] In this example, hES cells maintained in feederless culture media on laminin in conditioned medium were genetically modified by transfection with a plasmid carrying green fluorescent protein (GFP) induced by the CMV promoter.
[245] mEF conditioned medium was prepared as mentioned above. mEF was irradiated and inoculated at about 5.7 × 10 ⁴ cells / cm 2. After at least 16 hours, the medium was replaced with hES medium containing hbFGF added at 4 ng / ml. Conditioned medium was collected daily for feeding hES culture medium. Prior to addition to the hES culture medium, the medium was supplemented with additional 4 ng / ml of hbFGF. When stable transfection was required, mEF conditioned medium was supplemented with 200 μg / ml of Geneticin (Sigma catalog # G5013).
[246] H9 hES cells retained on the mEF feeder layer were recovered from the culture medium by incubation at 37 ° C. for 10 minutes using about 200 U / ml collagenase IV. Cells were isolated and resuspended in conventional hES culture medium or mEF conditioned medium. Cells in conventional media are then reinoculated into the mEF feeder layer and cells in mEF conditioned medium are Matrigel Or on laminin. The dentate density for all cultures was about 4 × 10 ⁴ cells / cm 2. Prior to transfection, cells on the matrix were maintained in conventional media while cells on the matrix were maintained for 1 or 2 days in mEF conditioned medium. Conditioned medium was changed every 24 hours.
[247] hES cell cultures were transfected with Lipofectamine 2000 ^™ described above. FACS analysis of GFP expression was performed as follows. HES cells were harvested using 0.5 mM EDTA in PBS and resuspended at about 1 × 10 ⁶ cells / test. Cells were washed with a solution containing PBS + 2% FBS, 0.1% sodium azide and 2 mM EDTA. SSEA-4 staining was performed in the same buffer using antibodies of dilution 1:15 obtained from Developmental Studies Hybridoma Bank (University of lowa, lowa City). Controls with the same isotype were purchased from Sigma (St. Louis MO, USA). Cells were incubated for 30 minutes at 4 ° C. with 100 μl of final volume, washed, and then 30 minutes at 4 ° C. with PE conjugated rabbit anti-mouse κ chain antibody (Becton Dickinson, San Jose, CA). Incubated for Samples were washed as described above and analyzed for GFP and SSEA-4 expression using CellQuest ^™ software on a FACScalibur ^™ flow cytometer (Becton Dickinson, San Jose, Calif.).
[248] hES cells of the H9 cell line retained on laminin in mEF conditioned medium were transfected with plasmids bearing GFP induced by the CMV promoter at 24 hours or 48 hours after dentate. Initial experiments used a mixture of 5 μg plasmid and 12 μl Lipofectamine 2000 ^™ . 1 ml of DNA / lipid complex was added to the cells, incubated 4 hours at 37 ° C. before addition of 3 ml of mEF conditioned medium, and GFP expression was monitored 24 hours after transfection.
[249] 4 shows the experimental results. Panel A: Morphology of H9 cells maintained on laminin. Panel B: GFP positive cells observed in the same colonies as seen in Panel A. Panel C: FACS analysis of the percentage of GFP positive cells in the SSEA-4 high population (undifferentiated cells). Cells were transfected 24 hours (bars 1 and 2) or 48 hours (bars 3 and 4) after inoculation and analyzed 24 hours (bars 1 and 3) or 48 hours (bars 2 and 4) after transfection It was. Bright green cells were observed in dense areas of undifferentiated ES colonies on laminin 24 hours after transfection (Panels A and B). Transfection at 48 hours after initial inoculation has the highest efficiency: 38% of cells are GFP positive as measured by FACS analysis 24 hours after transfection (Panel C).
[250] In subsequent experiments, Matrigel in mEF conditioned medium Or transfection efficiency of H9 cells retained on laminin-coded plates and cells retained on mEF feeders. Cells on the feeder layer maintained in conventional media were used as controls. Morphological differences between the cells on the feeder and the cells without the feeder were observed one or two days after inoculation. Colonies on the feeder were denser than cells maintained without the feeder layer; Individual hES cells in feederless culture medium were less dense and flat. There was no distinct difference in cell or colony form between cells on laminin and cells on Matrigel. These cells were transfected with plasmids expressing GFP induced by the CMV promoter two days after inoculation. 24 hours after transfection, cells were examined for GFP expression under fluorescence microscopy.
[251] Cells are placed on mEF feeder (mEF / RM) in conventional medium, on laminin (CM) in medium conditioned by mEF, or Matrigel in conditioned medium (Matrigel / CM). Bright green cells were observed in undifferentiated hES colonies of feeder-free culture media. In contrast, very few green cells were identified in the colonies on the feeder. FACS analysis, Matrigel It was shown that 16% of the cells in the phase and 14% of the cells on the laminin were GFP positive in the SSEA-4 high house, whereas only 5% of the cells were positive on the feeder. These results indicate that transfection efficiency is significantly higher by using feederless conditions.
[252] In the next experiment, the ratio of DNA: lipid; 2) addition of the DNA / lipid complex to the cells 4 hours prior to the addition of mEF conditioned medium and the addition of the complex to the cells in the presence of mEF conditioned medium; And 3) the effects on the use of Lipofectamine 2000 ^™ and FuGENE ^™ were evaluated.
[253] Transfections using Lipofectamine 2000 ^™ are described above. Transfection with FuGENE ^™ was performed as follows. Plasmid DNA (5-10 μg pEGFP-C1, ClonTech Catalog # 6084-1) was diluted with water to a final volume of 100 μl. In the experimental sample, the FuGENE ^TM ㎕ of 5-30 was adjusted to a final volume of 100 by adding a sufficient ㎕ OptiMEM ^TM. The DNA solution was then slowly added to the FuGENE ^™ solution and mixed gently. Prior to supplementing with 800 μl OptiMEM ^™ , the mixture was incubated at room temperature for 30 minutes. The cells were washed with 3 ml of pre-warmed OptiMEM ^™ and incubated for 4 hours at 37 ° C. in 1 ml of DNA / lipid mixture solution. In some experiments, an additional 2 ml of mEF conditioned medium is added to the wells at 4 hours, in other experiments a DNA / lipid mixture is added to a well containing 2 ml of mEF conditioned medium, and the cells are then overnight in the mixture. Incubated.
[254] The highest efficiency was obtained under the following conditions: Bar 1 = 5 ml of plasmid + 12 in which 1 ml of DNA / lipid mixture was added to a well containing 2.5 ml of mEF conditioned medium and the cells were incubated overnight in the mixture. Μl of a mixture of Lipofectamine 2000 ^™ . A mixture of 10 μg of plasmid + 15 μl of FuGENE ^™ incubated cells in 1 mL of DNA / lipid mixture for 4 hours before addition of bars 2 and 3 = 2.5 mL of mEF conditioned medium. L = Lipofectamine 2000 ^™ ; F = FuGENE ^™ .
[255] To test whether hES cells perform stable genetic modifications without a feeder, Matrigel H1 hES cells maintained in the stomach were cotransfected with a mixture of 7.5 μg of plasmid with β-galactosidase induced by EF1a promoter and 2.5 μg of plasmid with PGK promoter to induce neophosphorase gene I was. Matrigel in mEF conditioned medium Transfected 48 hours after wounding on the phase. 10 μg of plasmid + 15 μl of FuGENE ^™ were incubated with cells in 1 mL for 4 hours before adding 2.5 mL of mEF conditioned medium. After 48 hours, the medium was replaced with mEF conditioned medium supplemented with 200 μg / ml geneticin. Cultures were maintained in the genesis containing medium with daily medium replacement for at least 21 days. All of the mock transfected cultures (ie, providing FuGENE ^™ mixed with water rather than plasmid) died within 48-72 hours. Drug resistant colonies appeared in wells transfected with both FuGENE ^™ and plasmids at a frequency of about 1 per 10 ⁵ originally transfected cells. This colony was maintained and expanded in geneticin containing mEF conditioned medium.
[256] Example 9: Preparation of a vector in which the thymidine kinase gene is present under the control of the hTERT promoter sequence
[257] A lambda clone named λGΦ5 containing the hTERT promoter has been deposited in the American Type Culture Collection (ATCC) (US VI 20110 Manassas University Blvd. 1081) under accession number 98505. λGΦ5 contains an insertion of 15.3 kbp that includes about 13,500 bases upstream from the hTERT coding sequence.
[258] Not1 fragments containing the hTERT promoter sequence were subcloned into the Not1 site of a plasmid derived from pUC named pGRN142. Subclones (plasmid “pGRN140”) containing 9 kb NcoI fragments (with hTERT gene sequence and lambda vector sequence of about 4 to 5 kb) were partially sequenced to determine the direction of insertion. pGRN140 was cleaved with SalI to remove the lambda vector sequence, and the resulting plasmid (with the removed lambda sequence) was named pGRN144. The pGRN144 insert was then sequenced.
[259] SEQ ID NO: 1 is a list of sequence data obtained. Nucleotides 1-43 and 15376-15418 are plasmid sequences. Thus, genomic insertion begins at residue 44 and ends at residue 15375. The start of the cloned cDNA fragment corresponds to residue 13490. There is an Alu sequence element located about 1700 base pairs upstream. The sequence of the hTERT insert of pGRN142 can be obtained from GenBank (http://www.ncbi.nlm.nih.gov/) under the current accession number PGRN142.INS AF121948. The numbering of hTERT residues for the plasmids in the detailed description below begins with translation initiation codons, according to standard practice in the art. The hTERT ATG codon (translation initiation site) starts at residue 13545 of SEQ ID NO: 1. Thus, the first upstream position, position-1, corresponds to nucleotide 13544 of SEQ ID NO: 1.
[260] Fuming studies were performed with reporter constructs comprising various hTERT upstream regions and intron sequences. BgIII-Eco47III fragments were cut from pGRN144 (mentioned above) and cloned into the BgIII-NruI site of pSEAP2Basic (ClonTech, San Diego, Calif.) to produce a plasmid designated pGRN148. Plasmid pGRN150, a secondary reporter promoter, was prepared by inserting a BgIII-FspI fragment from pGRN144 into the BgIII-NruI site of pSEAP2. Plasmid pGRN173 was constructed using segments of EcoRV-StuI (+445 to -2482) from pGRN144. This includes a promoter reporter plasmid containing a hTERT promoter region from the upstream of the hTERT open reading frame, just after the first intron in the coding region, containing the starting Met codon of the hTERT open reading frame changed to Leu. Make. Plasmid pGRN175 was prepared by APA1 (Klenow blunt) -SRF1 cleavage and ligation of pGRN150 to delete most of the genomic sequence upstream of hTERT. This creates a promoter / reporter plasmid using 204 nucleotides (position of -117 in -36) of the hTERT upstream region sequence. Plasmid pGRN176 was prepared by PML-SRF1 relocation of pGRN150 to delete most of the hTERT upstream region sequence. This creates a promoter / reporter plasmid using 204 nucleotides (positions -36 to -239) of the hTERT upstream region sequence.
[261] The activity level of secretory placental alkaline phosphatase (SEAP) was detected using the chemifluorescent substrate CSPDTM (ClonTech). SEAP activity detected in culture media was found to be directly proportional to the change in the intracellular concentration of SEAP mRNA. pGRN148 and pGRN150 plasmids (hTERT promoter-reporter) and pSEAP2 plasmids (containing positive control, SV40 initial promoter and enhancer) were transfected with the test cell line. Both pGRN148 and pGRN150 constructs induced SEAP expression as efficiently as SEAP2 in immortal (tumor inducible) cell lines. Only the pSEAP2 control showed detectable activity in non-immortal cells.
[262] The ability of the hTERT promoter to specifically induce the expression of thymidine kinase ( tk ) genes in tumor cells was examined using the following various constructs. One construct named pGRN266 contains an EcoRI-FseI PCR fragment with the tk gene cloned into the EcoRI-FseI site of pGRN263. PGRN263, containing about 2.6 kb of hTERT promoter sequence, is similar to pGRN150 but contains the neomycin gene as a selection marker. pGRN267 contains an EcoRI-FseI PCR fragment with the tk gene cloned into the EcoRI-FseI site of pGRN264. PGRN264, containing about 210 bp of hTERT promoter sequence, is similar to pGRN176 but contains the neomycin gene as a selection marker. pGRN268 contains an EcoRI-XbaI PCR fragment with the tk gene cloned into the EcoRI-XbaI (not methylated) site of pGRN265. PGRN265, containing about 90 bp of hTERT promoter sequence, is similar to pGRN175 but contains neomycin as the selection marker.
[263] These hTERT promoter / tk constructs, pGRN266, pGRN267 and pGRN268, are reintroduced into mammalian cells and tkl + stable clones (and / or mass populations) are selected. In vitro livercyclovir treatment of tkl + cells results in selective destruction of all tumor cell lines tested, including 143B, 293, HT1080, Bxpc-3 ', DAOY and NIH3T3. Gancyclovir treatment does not work for normal BJ cells.
[264] 5 is a map of TPAC adenovirus pGRN376. It was prepared by cloning the NOT1-BAMH1 fragment from pGRN267 to the NOT1-BGL2 site of pAdBN (Quantum Biotech). The 7185 bp vector contains the herpes simplex thymidine kinase (TK) gene under the control of the medium length hTERT promoter sequence.
[265] Example 10 Transduction of hES Cells by Thymidine Kinase Constructs
[266] These experiments examine the effect on hES cells of the pGRN376 vector mentioned in the previous example. The vector contains the herpes virus thymidine kinase gene under the control of the telomerase reverse transcriptase promoter. The expression of the thymidine kinase gene in cells is susceptible to toxicity from prodrug gancyclovir.
[267] Undifferentiated H1 cells were plated into 24 well plates (one confluent well of a 6 well plate was divided into wells of a 24 well plate). After 48 hours, some wells were infected with TAP vectors of MOI 30 or 100. 4 hours after virus vector addition, the medium is replaced with fresh mouse embryonic fibroblast conditioning medium (mEF-CM); Some wells were replaced with medium supplemented with 30 μM Gancyclovir (GCV). Cells exposed to GCV were resupplied daily for 4 days with mEF-CM containing 30 μM of GCV. Two, three and four days after the start of the GCV treatment, the wells were harvested and analyzed by flow cytometry to assess changes in 1) total cell number and 2) cell viability (measured by PI exclusion).
[268] 6 shows the results of the experiment. No change in total cell number was detected in MOI 30 in the absence of GCV; There was a slight decrease in MOI 100 in the absence of GCV starting at 48 hours. Evidence for the toxicity of GCV itself was detected: Wells treated with GCV alone contained about 55% of the cells in control wells on day 2 and decreased to 40% by day 4. Equivalent results were obtained in wells treated with GRN376 of MOI 30 or 100 incubated in the presence of GCV: By day 2, the wells contained 18% of the cells contained in the control wells, whereas on days 3 and 4 Wells contained 6% and 8% of control well cells.
[269] On day 4 in the absence of GCV there was some toxicity at MOI 100 (50% cells in ES gate, 83% for control cells). Some toxicity of GCV alone was 75% cells (control 85%) in ES gate on day 2; On day 3, 68% (control 82%); On day 4, 50% (control 65%) were observed. Wells treated with GRN376 with MOI 30 or 100 cultured in the presence of GCV showed similar results: By day 2, these wells contained 24-28% cells in the ES gate; On day 3 they contained 19-22% cells in the ES gate; On day 4 these wells contained 12% cells in the ES gate. Thus, GRN376 + GCV is effective at killing undifferentiated hES cells at MOI as low as 30.
[270] Grinding experiment
[271] Undifferentiated H1 cells were plated into 24 well plates (one confluent well of 6 well plate was divided into 24 wells of 24 well plate). After 48 hours, some wells were infected with pGRN376 of MOI 30. Four hours after addition of the viral vector, the medium was replaced with fresh mEF-CM; Some wells were replaced with medium supplemented with 5, 10, 20, 30 or 40 μM of gancyclovir (GCV). Cells exposed to GCV were resupplied daily for 2 days with mEF-CM containing GCV. Two days after the start of the GCV treatment, wells were harvested and analyzed by flow cytometry to assess changes in total cell number and cell viability (measured by PI exclusion).
[272] 7 shows the results of the experiment. About 20 μM of GCV was optimal under the test conditions.
[273] Comparison with other hES cell lines
[274] Undifferentiated hES of the cell lines designated H1 and H7 cells were plated into 24 well plates (one confluent well of a 6 well plate was divided into 24 wells of a 24 well plate). After 48 hours, some wells were infected with pGRN376 of MOI 30. 4 hours after virus vector addition, the medium was replaced with fresh mEF-CM; Some wells were replaced with medium supplemented with 20 μM of GCV. Cells exposed to GCV were resupplied daily for 3 days with mEF-CM containing GCV. Four days after the start of GCV treatment, wells were harvested and analyzed by flow cytometry to assess changes in total cell number.
[275] 8 shows the result. After TPAC vector treatment, total cell numbers showed a decrease in cell numbers in both cell lines. H7 showed lower toxicity induced by GCV than H1. Thus, other hES cell lines respond to TPAC vectors. In later studies, H9 cell lines were also found to be very sensitive to GCV after TPAC vector treatment.
[276] Example 11: Selection of Differentiated Cells
[277] In this experiment, hES cells were treated with retinoic acid (RA) or dimethyl sulfoxide (DMSO), followed by expression of hTERT and OCT-4 after TPAC treatment.
[278] Undifferentiated H1 cells were plated in 24-well plates (one confluent well of a 6 well plate was divided into 24 wells of a 24 well plate). After 24 hours, some wells were reloaded with mEF-CM containing 500 nM RA or 0.5% DMSO; For the remainder of this experiment, wells were replenished with medium supplemented with RA or DMSO. Seven days after treatment with RA or DMSO, cells were infected with GRN376 of MOI 30.
[279] 4 hours after virus vector addition, the medium is replaced with fresh mEF-CM medium (+ RA or DMSO appropriate amount); In addition, some wells were replaced with medium supplemented with 20 μΜ Gancyclovir (GCV). Cells exposed to GCV were replenished daily with mEF-CM medium containing GCV for 3 days. Three days after the start of GCV treatment, the wells were harvested and analyzed by flow cytometry to assess changes in total cell number. Additional wells were used to culture any remaining undifferentiated stem cells; The medium of the wells was changed to mEF-CM (without RA, DMSO or GCV). Cells were resupplied with mEF-CM daily for 7 days and recovered for RNA isolation. These samples were analyzed by quantitative RT-PCR for hTERT and OCT-4 expression.
[280] 9 shows the cell number decreases after TPAC treatment. After 7 days of drug pretreatment with TPAC + GCV, all wells contained similar cell numbers. During incubation of viable stem cells, the wells were confluent with cells that appeared to be significantly differentiated; Undifferentiated hES cells were not pronounced. Wells containing cells pretreated with RA were clearly distinguished from the appearance of cells pretreated with mEF-CM free of impurities or cells pretreated with mEF-CM + DMSO. RT-PCR analysis (bottom panel, non-quantitative, 35 cycles) revealed that OCT-4 expression was not detectable in viable cells from mEF-CM or DMSO treated wells, whereas two of four RA pretreated samples were highly It showed weak OCT-4 PCR product.
[281] Thus, detectable undifferentiated cells do not survive subsequent cultures of wells grown in mEF-CM or mEF-CM + DMSO after TPAC treatment. RA pretreatment leads to the detection of low levels of OCT-4 in viable cells. It is unclear whether this reflects the persistence of undifferentiated stem cells or the induction of OCT-4 expressing or other cell types.
[282] Example 12 Stem Cell Lines Containing TPAC Constructs
[283] To facilitate the generation of stable cell lines genetically modified by the hTERT promoter / thymidine kinase construct, plasmid pGRN376 (Example 9) was modified to remove most of the adenovirus sequence. The plasmid was digested with StuI and NotI, then bloated and religated. The remaining vector contained the hTERT promoter / thymidine kinase construct in front of the transcription blocking sequence that inhibits the promotion of tk gene expression by region upstream from the promoter sequence from an integration site in the genome. The modified vector was named pGRN376mod.
[284]
[285] As mentioned above, the culture of H9 hES cell line was split 1: 4 using 0.5 mM EDTA containing PBS and placed in feeder-free culture medium in 6-well plate. After 24 hours, as described above, FuGENE ^™ -6 was used to cotransfect the cells with a plasmid encoding 2 μg of GRN376mod + 0.5 μg of neomycin phosphotransferase. A medium containing 200 μg / μL geneticin was added 48 hours after transfection to select transfected cells. After 7-10 days in the Geneticin containing medium, 103 individual colonies were picked from the wells, individually placed in a 24-well plate, and then expanded by incubating for about 7 days. 90 individual clones were screened for sensitivity to prodrug gancyclovir at a concentration of 30 μM. Within 1-3 days of incubation with gancyclovir, essentially all undifferentiated cells were killed, identifying 10 clones in which only the remaining cells with differentiated appearance remained the main colonies.
[286] In another experiment, the H1 cell line of hES cells (passage 59) was converted to Matrigel in standard hES cell medium (0 days; MEF conditioned medium containing 1 ml / well, 4 ng / ml). Split 1: 6 in 24-well plates coated with. The medium was replaced with fresh standard medium on the following date. On day 3, the medium was removed and replaced with 200 μl / well of standard medium (Mok transfection), or pGRN376 virus of MOI 100 diluted with 200 μl / well of standard hES medium. After 4 hours, the medium was removed from all wells and replaced with 1 mg / ml standard hES medium with or without 30 μM gancyclovir. On each day for 3 days thereafter, the wells were replaced with fresh medium (with or without 30 μM of gancyclovir).
[287] 10 is a micrograph of cells at day 6. In wells transduced with control vectors (Panel A), hES colonies formed colonies with general characteristics. In wells transduced with pGRN376 virus and then treated with gancyclovir (Panel B), most or all ES cell colonies were killed, leaving only differentiated cells. TPAC treated wells contained 8 times less cells than control wells.
[288] Example 13: Further Characterization of Stable TPAC Embryonic Stem Cell Lines
[289] TPAC stable colonies, Matrigel reduced growth factor HES cell line H9 grown in mEF conditioned medium with basal FGF on a plate coated with was induced by cotransfection of pGRN376mod and pGK-neo plasmids.
[290] In one experiment, the H9 cell line (p 80) was passaged six times using 0.5 mM EDTA before transfection. 2 μg pGRN376m plasmid and 0.5 μg pGK-neo plasmid were used to transfect each well of a 6 well plate of ES cells using a 3: 2 ratio of FuGENE ^™ (lipid-based transfection promoter) to DNA. It was. Cells were transfected 24 hours after inoculation and G418 selection was started 24 hours after transfection. 101 G418 resistant colonies were picked using collagenase. Clones from cells transfected with pGK-neo alone were isolated as controls for cotransfection.
[291] For primary and secondary GCV screening, individual colonies were expanded using 0.5 mM EDTA. Primary screening (week 1 and week 2) by 30 μM GCV identified 10 GCV sensitive clones and 20 partial GCV sensitive clones. Second screening (3 weeks and 4 weeks) by 30 μM GCV identified 9 GCV sensitive clones, including those designated H9-376m-18, H9-376m-77 and H9-376m-62 . After the second GCV screening, cells were expanded to bulk culture using 0.5 mM EDTA and then passaged using collagenase.
[292] In the second experiment, the H9 cell line (p 24) was initially passed using 0.5 mM EDTA and then transfected as above. 62 G418 sensitive cotransfected colonies were picked using collagenase on day 0. Primary screening with 30 μΜ GCV (day 20, 22 and 26) identified one GCV sensitive clone and four partial GCV sensitive clones. After primary GCV screening, cells were expanded to bulk culture using 0.5 mM EDTA and gradually recovered to standard collagenase passages. Secondary screening with 30 μΜ GCV identified one GCV sensitive clone (designated H9-376m-6).
[293] For GCV sensitive clones from each of these experiments, additional selection was performed by incubation with G418. Clones designated H9-376m-18 and H9-376m-77 showed unstable G418 resistance. Clones named H9-376m-62, H9-376m-6 and H9-pGK-neo-1 were G418 resistant.
[294] 11 (top) shows the sensitivity of stable TPAC cell lines to gancyclovir (GCV). Each cell line was exposed to reduced amounts of GCV to determine the minimum concentration of GCV required to produce complete killing of undifferentiated ES cells with a minimal amount of toxicity. To kill undifferentiated ES cells similar to 30 μM but with significantly lower toxicity, concentrations as low as 0.5 μM were measured.
[295] FIG. 11 (bottom) shows the sensitivity of TPAC cell line to (E) -5- (2-bromovinyl) -2′-deoxyuridine (BVDU) with another prodrug. Although a significant decrease in cell count was observed at all BVDU concentrations examined, ES cell death was not observed accurately. The number of cells present in high concentrations of BVDU does not decrease to the blank control level.
[296] To demonstrate the expression of HSV-TK in stable ES cell lines, from HS9-376m-6, H9-376m-62 and H9-pGK-neo-1 cultures, using HSV-TK or OCT-4 specific primers RT-PCR was performed on undifferentiated cells. TK expression was detected in H9-376m-6 and H9-376m-62 samples, but not in high concentrations of RNA in H9-pGK-neo samples, while OCT-4 expression was detected in all samples.
[297] Example 14 Promoter Specificity for Undifferentiated Cells
[298] To determine hTERT promoter specificity in stable TPAC clones, GCV sensitivity was measured after differentiation of each of the TPAC clone and the pGK-neo control clone.
[299] Undifferentiated Cells, Matrigel with Reduced Growth Factor In plates coated with, inoculated without bFGF with KO DMEM + 20% Hyclone FBS (differentiation conditions). The culture was maintained for 7 days under differentiation conditions, passaged and then placed under differentiation conditions for an additional 4 days. After the above treatment, the culture medium contained almost none of any undifferentiated cells (assessed by morphology). At this point, 30 μM of GCV was added to the differentiated cultures for 4 days.
[300] Sensitivity to GCV was observed in differentiated cultures obtained from TPAC cell lines H9-376m-6, H9-376m-62, H9-376m-18 or H9-376m-77, or transfected control cell line H9-pGK-neo. It wasn't. Thus, in integrated constructs, hTERT has adequate sensitivity to actuate the TK effector gene only in undifferentiated cells.
[301] Example 15 Expression of ES Markers in Undifferentiated TPAC Cell Lines
[302] Expression of ES markers on stable TPAC ES cell lines was measured by flow cytometry. Cells were recovered from confluent cultures using 0.5 mM EDTA, monoclonal antibodies against human SSEA-4, SSEA-1, Tra-1-60, Tra-1-81, CD9, AC133 and appropriate fluorine. Incubation was with co-conjugated secondary antibody. Expression levels were measured using FACSCalibur ^™ . Negative controls matching the appropriate isotype and viability assessment were used to determine the level of nonspecific binding.
[303] Markers for hES Parent Cells and Stable TPAC Cell Lines Phenotype MarkerH9P 35H9-376m-62P 80 + 15H9P 25H9-pGK-neop 24 + 16H9-376m-6p 24 + 19H9-376m-62p 80 + 27 SSEA4 +92 *10064379198 SSEA1 +One00000 Tra-1-60 +989989898690 Tra-1-81 +969982887797 CD9 +889581989199 AC133 +908172786870 * Is the percentage of viable cells expressing the marker
[304] H9-376m-6, H9-376m-62, and H9-pGK-neo-1 cell lines had expression of all tested ES markers at levels comparable to untransfected H9 cell lines.
[305] Example 16: Activity of TPAC Cell Lines Performing Proper Differentiation
[306] For H9-376m-6, H9-376m-62, H9-376m-77, H9-376m-18 and H9-pGK-neo-1, the ability to generate cells from three embryonic lines was evaluated. Cultures (EB) were made from each cell line and incubated in suspension containing KO DMEM and 20% Hyclone FBS. After 4 days of culture, EBs were dentured on gelatin-coated chamber slides and grown for an additional 8-10 days. Cultures were assessed for the presence of beating cells and stained during the presence of β-tubulin, AFP, muscle specific actin and cardiac troponin I positive cells.
[307] 12 is a representative fluorescence micrograph of immunocytochemistry. The results were as follows:
[308] H9-376m-18 p 80 + 20 could produce large cultures (one experiment). Beating area (consistent with cardiomyocyte lineage cells) was observed. Muscle specific actin +++; α-fetoprotein +++; β-tubulin +++; Cardiac troponin I +.
[309] H9-376m-77 p 80 + 16 produced only small cultures (one experiment). No beating area was observed. Muscle specific actin +; α-fetoprotein +; β-tubulin-(not detected); Cardiac troponin I-.
[310] H9-376m-62 p 80 + 16, p 80 + 20, p 80 + 21, p 80 + 24 could only produce small cultures (4 experiments). No beating area was observed. Muscle specific actin ++; α-fetoprotein +; β-tubulin +; Cardiac troponin I-.
[311] H9-376m-6 p 24 + 12, p 24 + 13, p 24 + 21 could form large cultures (3 experiments). Many beating areas were observed in all experiments. Muscle specific actin ++; α-fetoprotein ++; β-tubulin ++++; Cardiac troponin I +.
[312] H9-pGK-neo-1 p 24 + 9, p 24 + 10, p 24 + 13 could form large cultures (three experiments). The beating area was observed twice in three experiments. β-tubulin +++; Cardiac troponin I +; Muscle specific actin ++++; α-fetoprotein ++.
[313] The results of Examples 13-16 show that at least three of the stem cells containing the thymidine kinase gene induced by telomerase can be differentiated into individual cells of three germ layers. Cells differentiated from these cell lines contain important stop-gaps for residual or reexpressing undifferentiated cells. Gancyclovir at concentrations as low as 2.5 μM can kill all of these modified undifferentiated ES cells in about 4 days.
[314] Sequence data
[315] Sequences Listed Here SEQ ID NO:designationReference OneLambda clone designated ATG Access 5 (ATCC Accession No. 98505), which contains the human telomerase reverse transcriptase (hTERT) genome insert (residues 44-15375) .- The ATG translational initiation starts at residue 13545.GenBank Accession No. AF121948 International Patent Publication WO 00/46355 2Sequence of thymidine kinase and 3KBL gene of herpes simplex virus type 1Gene Bank Accession JO2224, McKnight et al., Nucleic Acids Res. 8: 5949 (1980); Wagner et al., Proc. Natl. Acad. Sci. U.S.A. 78: 1441 (1981) 3Amino acid sequence of thymidine kinase and 3 KBL of herpes simplex virus type 1(Same as above) 4-7Probes and PrimersArtificial sequence
[316] It will be appreciated that the compositions and procedures provided in the above detailed description can be effectively modified by those skilled in the art without departing from the scope of the invention as set forth in the claims below.

权利要求:
Claims (30)
[1" claim-type="Currently amended] A population of cells differentiated from primate differentiated pluripotent stem (pPS) cells, characterized by essentially no undifferentiated cells.
[2" claim-type="Currently amended] 2. The nucleic acid molecule of claim 1, wherein said cell comprises a nucleic acid molecule comprising a P-X structure, wherein X is lethal to the expressing cell, or is a nucleic acid sequence encoding a product that makes the expressing cell susceptible to a lethal effect of an external agent; P is a transcriptional control element that biases X in undifferentiated cells.
[3" claim-type="Currently amended] Nucleic acid molecules comprising a P-X structure, wherein X is lethal to the expressing cell or is a nucleic acid sequence that makes the expressing cell susceptible to a lethal effect of an external agent; P is a transcriptional control element that biases expression of X in undifferentiated cells.
[4" claim-type="Currently amended] 4. A cell or population of cells according to claim 2 or 3, wherein X encodes a toxin or a protein that induces or mediates apoptosis.
[5" claim-type="Currently amended] 4. A cell or population of cells according to claim 2 or 3, wherein X encodes an enzyme that converts the prodrug into a compound that is lethal to X expressing cells.
[6" claim-type="Currently amended] 6. A cell or population of cells according to claim 5, wherein X encodes thymidine kinase.
[7" claim-type="Currently amended] The cell or population of cells according to any one of claims 2 to 6, wherein the P-X is an introduced heterologous molecule.
[8" claim-type="Currently amended] 7. A cell or population of cells according to any of claims 2 to 6, wherein P is an endogenous transcriptional control element.
[9" claim-type="Currently amended] 7. A cell or population of cells according to any one of claims 2 to 6, wherein P is a promoter of an OCT-4 promoter or telomerase reverse transcriptase (TERT).
[10" claim-type="Currently amended] 10. The cell or cell population according to claim 1, wherein said stem cells are human embryonic stem (hES) cells.
[11" claim-type="Currently amended] A method of producing a differentiated cell population comprising the steps of:
a) a nucleic acid molecule comprising the structure P-X, wherein X is lethal to the expressing cell or is a nucleic acid sequence encoding a product which makes the expressing cell susceptible to a lethal effect of an external agent; P is a transcriptional control element that biases X in undifferentiated cells, providing a population of cells comprising undifferentiated stem cells;
b) inducing differentiation of at least some undifferentiated cells in said population.
[12" claim-type="Currently amended] 12. The method of claim 11, further comprising binding said cell population with an external agent.
[13" claim-type="Currently amended] Nucleic acid molecules comprising a P-X structure, wherein X is a nucleic acid sequence encoding a product that is lethal to an expressing cell; P is a transcriptional control element that biases X in undifferentiated cells. Genetically modifying undifferentiated stem cells in said population.
[14" claim-type="Currently amended] 14. The method of any one of claims 11 to 13, wherein X is a toxin or a protein that induces or mediates apoptosis.
[15" claim-type="Currently amended] A method of depleting a cell population of undifferentiated stem cells, characterized by the following steps:
a) a nucleic acid molecule comprising a P-X structure, wherein X provides a cell that is susceptible to lethal effects of an external agent upon expression; P is a genetically modified undifferentiated stem cell in said population so that it is a transcriptional control element that biases X in undifferentiated cells;
b) depleting undifferentiated cells from said population by combining said cells with an external agent.
[16" claim-type="Currently amended] 16. The method of any one of claims 11, 12 and 15, wherein X is an enzyme encoding an enzyme that converts the prodrug into a compound that is lethal to X expressing cells.
[17" claim-type="Currently amended] The method of claim 16, wherein X encodes thymidine kinase.
[18" claim-type="Currently amended] 18. The method of claim 16 or 17, wherein the external agent is ganciclovir.
[19" claim-type="Currently amended] 19. The method of any one of claims 11 to 18, wherein P-X is a heterologous molecule introduced.
[20" claim-type="Currently amended] 19. The method of any one of claims 11 to 18, wherein P is an endogenous transcriptional control element.
[21" claim-type="Currently amended] 21. The method of any one of claims 11 to 20, wherein said population is genetically modified such that X is routinely expressed in undifferentiated cell cells in said population.
[22" claim-type="Currently amended] 21. The method of any one of claims 11 to 20, wherein P-X is inherited into progeny cells of cells of said population and expressed in undifferentiated progeny cells.
[23" claim-type="Currently amended] 23. The method of any one of claims 11 to 22, wherein P is a promoter of the OCT-4 promoter or telomerase reverse transcriptase (TERT).
[24" claim-type="Currently amended] 24. The method of any one of claims 11 to 23, wherein said nucleic acid molecule contains a P-X-Y structure, wherein Y is a drug resistance gene.
[25" claim-type="Currently amended] 25. The method of any one of claims 11 to 24, wherein said stem cells are human embryonic stem (hES) cells.
[26" claim-type="Currently amended] A population of differentiated cells, characterized in that it is obtained according to the method of any one of claims 11-25.
[27" claim-type="Currently amended] 27. A cell according to any one of claims 1 to 10 and 26, characterized in that it is a population of neurons, astrocytic cells, oligodendrocytes, hepatocytes, cardiomyocytes, osteoblasts or their committed precursor cells. Cell population.
[28" claim-type="Currently amended] A pharmaceutical formulation for human or animal surgery or treatment, characterized in that it comprises a cell or cell population according to any one of claims 1 to 10 and 26 to 27.
[29" claim-type="Currently amended] P-X structure, wherein X is a nucleic acid sequence encoding a product that converts a prodrug into a compound that is lethal to undifferentiated intermediate cells; P is a transcriptional control element that biases X in undifferentiated cells.] The use of a prodrug in the manufacture of a medicament for depleting undifferentiated stem cells in a human or animal body.
[30" claim-type="Currently amended] 30. The use according to claim 29, wherein said prodrug is ganciclovir.

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Gai et al.2009|Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts

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Laflamme et al.2005|Formation of human myocardium in the rat heart from human embryonic stem cells

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Sanvito et al.1994|TGF-beta 1 influences the relative development of the exocrine and endocrine pancreas in vitro

同族专利:

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公开号 | 公开日

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CA2434760A1|2002-05-30|

---

CA2434760C|2013-09-24|

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WO2002042445A3|2003-01-16|

---

GB2386120B|2005-03-09|

---

IL155695D0|2003-11-23|

---

ES2544942T3|2015-09-07|

---

CN1478145A|2004-02-25|

---

US9023645B2|2015-05-05|

---

US8426198B2|2013-04-23|

---

US20020098582A1|2002-07-25|

---

EP1337632A2|2003-08-27|

---

US20030040111A1|2003-02-27|

---

WO2002042445A2|2002-05-30|

---

EP1337632B1|2015-05-20|

---

GB2386120A|2003-09-10|

---

GB0313389D0|2003-07-16|

---

JP2004523217A|2004-08-05|

---

US20130273651A1|2013-10-17|

---

US20060134782A1|2006-06-22|

---

IL155695A|2007-10-31|

---

AU3768102A|2002-06-03|

---

CN101928692A|2010-12-29|

---

AU2002237681B2|2007-03-22|

---

US6576464B2|2003-06-10|

引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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法律状态:
2000-11-27|Priority to US25335700P

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2000-11-27|Priority to US25344300P

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2000-11-27|Priority to US60/253,443

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2000-11-27|Priority to US60/253,357

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2001-02-13|Priority to US09/783,203

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2001-02-13|Priority to US09/783,203

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2001-11-26|Application filed by 제론 코포레이션

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2001-11-26|Priority to PCT/US2001/044309

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2003-10-17|Publication of KR20030081334A

优先权:

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申请号 | 申请日 | 专利标题

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US25335700P| true| 2000-11-27|2000-11-27|

---

US25344300P| true| 2000-11-27|2000-11-27|

---

US60/253,443|2000-11-27|

---

US60/253,357|2000-11-27|

---

US09/783,203|2001-02-13|

---

US09/783,203|US6576464B2|2000-11-27|2001-02-13|Methods for providing differentiated stem cells|

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PCT/US2001/044309|WO2002042445A2|2000-11-27|2001-11-26|Differentiated cells suitable for human therapy|